摘要
本文提出了一种偶合反应伏安酶联免疫分析测定人血清乙型肝炎Ｅ抗原（ＨＢｅＡｇ）的新方法。本法对酶标乙型肝炎Ｅ抗体测定的灵敏度比经典的ＥＬＩＳＡ显色光度法高２０倍。对ＨＢｅＡｇ的测定采用双抗体夹心法，对ＨＢｅＡｇ阳性对照血清的测定范围为１：１～１：１０２４，灵敏度比ＥＬＩＳＡ显色光度法高１６倍。用所建立的方法对实际病人血清样品进行了测定，并与现行的ＥＬＩＳＡ显色光度法进行对照，两种方法相关性很好。
A new voltammetric enzyme immunoassay with coupling reaction for hepatitis B E-antigen(HBeAg)determination in human sera is described.The reaction of ortho-tolidine (OT)with hydrogen peroxide catalyzed by horseradish peroxidase-labeled hepatitis B E-antibody(HBeAb-HRP)is coupled with the subsequent electrochemical reduction of OT oxidation product. The HBeAb-HRP activity can be measured, since the latter reaction exhibits a sensitive second-order derivative linear sweep voltammetric wave at -0.58V (SCE)in the Britton-Robinson (BR) buffer.The sensitivity of the proposed method for HBeAb-HRP detection is 20 times higher than the ELISA method.A double-antibody sandwich immunological method has been adopted for HBeAg determination.The linear range for HBeAg-positive contrast sera dilution is 1:1～1:1024.Its sensitivity is 16 times higher than the conventional ELISA method taken as a control method. The agreement between the methods is good.
出处
《分析测试学报》
CAS
CSCD
1996年第2期34-38,共5页
Journal of Instrumental Analysis
基金
国家自然科学基金
