摘要
目的观察MKK7/JNK途径对HCT116细胞恶性生长的影响。方法建立稳定敲低MKK7与JNK的细胞株,Western印迹实验检测JNK与MKK7的表达,集落形成技术检测HCT116细胞体外增殖。通过裸鼠荷瘤实验分析稳定敲低MKK7与JNK细胞株体内恶性生长能力。结果稳定敲低MKK7与JNK细胞株集落形成能力显著降低,成瘤能力明显减弱。结论 MKK7/JNK途径促进了HCT116细胞恶性生长。
Objective To observe the effect of MKK7/JNK pathway on the oncogenic growth of HCT116 cells.Methods The established HCT116 single clones which stably expressed control short hairpin RNA（shRNA）,JNK shRNA,or MKK7 shRNA and the efficiency of gene silencing was confirmed by Western blotting analysis.The oncogenic growth of HCT116 cells in vitro and in vivo was examined with colony formation assay and nude mice inoculated with stable clones.Results The ability of colony formation and tumorigenicity in nude mice was significantly reduced in HCT116 cells with stable knock down of MKK7 or JNK.Conclusion The basal MKK7 / JNK activity promotes the oncogenesis growth of HCT116 cells.
出处
《军事医学》
CAS
CSCD
北大核心
2013年第9期666-670,共5页
Military Medical Sciences
基金
国家自然科学基金资助项目(30973547
31100544)
