摘要
目的。建立检测食品中甜蜜素的毛细管柱气相色谱分析方法，优化实验条件，获得理想的灵敏度、精密度和重现性。方法：根据甜蜜素衍生反应机理，控制反应温度、反应试剂用量、提取时间以及气相色谱条件，以毛细管柱气相色谱检测。结果：在30℃反应温度下，漩涡提取150S，在0．02～O．2mg／mL范围内，衍生物的峰面积与甜蜜素浓度线性良好，r=0．9997，检出限为1．1μg，平行测定峰面积RSD为1．2％，重复测定峰面积RSD为2．1％。结论：该方法经优化条件，简单、快速，干扰少，灵敏度、精密度高，重现性好，适用于甜蜜素的检测分析。
Objective： To establish a method for detecting sodium cyclamate in food by Capillary Column Gas Chromatography, and get satisfactory sensitivity, precision and repeatability by optimizing the experimental conditions. Method. Based on the derivatization reaction mechanism sodium cyclamate, the reaction temperature, reagent dosage, extraction time and chromatographic conditions are controlled to improve the effect. Result. The peak areas of derivatives are in regular linear relationship （r=0. 9997） with the mass concentration of sodium cyclamate within the scope of 0.02-0.2 mg/ mL. The detection limit is 1.1 μg and the RSDs of peak areas for precision and repeatability are 1.2% and 2.1%. Conclusion： The method is simple, rapid, with less interference, high sensitivity, precision and repeatability, thus it is suitable for detection and analysis of sodium cyclamate.
出处
《中国调味品》
CAS
北大核心
2013年第11期81-83,102,共4页
China Condiment
关键词
气相色谱法
毛细管柱
甜蜜素
Gas Chromatography
Capillary Column
sodium cyclamate
