摘要
目的 :采用新型高取代磺酸基环糊精为毛细管电泳手性选择剂 ,研究萘普生、酮洛芬、氧氟沙星和阿托品 4种药物的对映体分离。方法 :在毛细管电泳仪上 ,以逆向区带电泳模式 ,研究 3种高取代磺酸基环糊精手性选择剂对 4种药物对映体拆分的影响 ,优化了分离条件 ,并探讨了分离机理。结果 :在优化的条件下 ,4种药物对映体取得很好的分离结果。结论 :在毛细管电泳中 ,高取代磺酸基环糊精是一种很有潜力的手性选择剂 ,值得进一步研究。
Objective:To investigate the separation of the enantiomers of four drugs-naproxen,ketoprofen,ofloxacin and atropine by capillary electrophoresis (CE)using highly sulfated cyclodextrins(HS-CDs) as chiral additives.Methods:On neutral fused silica capillary column,using triethylammonium phosphate solution(pH 2 5)as background electrolyte,and HS-α-CD,HS-β-CD,HS-γ-CD as chiral additives,capillary zone electrophoresis with the reversed-polarity mode was applied to examine the effect of the HS-CD type on the enantioseparation of the four drugs.Results:The optimal chiral selectors for ketoprofen,ofloxacin and atropine are HS-β-CD,and HS-γ-CD for naproxen.Base-line separation of the enantiomers of the four drugs were all obtained under their respective optimal CE conditions.Conclusion:HS-CDs are potential powerful chiral selectors in CE,which are worth developing further.
出处
《药物分析杂志》
CSCD
北大核心
2000年第6期363-366,共4页
Chinese Journal of Pharmaceutical Analysis
基金
天津市自然科学基金
高校"211工程"项目
关键词
高取代磺酸基环糊精
CZE
手性选择剂
药物对映体
enantiomers
chiral separation
highly sulfated cyclodextrins
capillary electrophoresis
