摘要
目的：考察酮洛芬（Ketoprofen,KT）对映体在多糖衍生物手性固定相的色谱行为,建立酮洛芬对映体的分离分析方法,并用于血浆样品中酮洛芬对映异构体的测定。方法：色谱柱Chiralpak AD（250mm×4.6mm,10μm,Daicel Chemicals）,流动相组成为正己烷：异丙醇：TFA（80：20：0.02）,检测波长254nm,流速1.0ml/min,柱温为25℃。结果：酮洛芬对映体在10min内实现分离,分离度大于2.0,对映体R和S分别在血药浓度0.1-10μg/ml范围内线性关系良好R-（-）（r=0.9998）,S-（＋）（r=0.9996）,单一对映体的最低检测限为2ng（S/N=3）。结论：建立了用ChiralpakAD柱分离分析酮洛芬对映异构体的方法,可用于单一对映异构体的光学纯度检测及药理学研究。
Objective：The separation effect for the enantiomers of ketoprofen on four diferent chiral columns was investigated and established a sensitive and stereospecific HPLC separation and determination method for ketoprofen enantiomers in animal plasma.Methods：The enantiomers of ketoprofen was resolved in the normal phase mode by using n-hexane∶isopropanol∶TFA（80∶20∶0.02）as mobile phase with UV detector at a wavelength of 254 nm.The flow rate was 1.0 ml/min.Results：Linear calibration curves were obtained in the range 0.1～10μg/ml for R-（＋）-ketoprofen（r=0.999 8）and S-（＋）-ketoprofen（r=0.999 6）in plasma.The detection limit（S/N=3） was 2 ng for each enantiomer.Conclusion：This method is simple,fast and can be used to detect optical purity of S-（＋）-ketoprofen and monitor pharmacokinetic studies of ketoprofen enantiomers.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2010年第4期586-590,共5页
Journal of Chongqing Medical University
