摘要
从 16个采自新疆不同棉区的棉花黄萎病菌 (Verticilliumdahliae)菌株和 3个落叶型与非落叶型对照菌株上共获得 2 2 5个不能利用硝酸盐的营养体突变株 (nitmutants)。经不同氮源利用的鉴定 ,89 33%属于nitA ,9 33%属于nitB ,1 33%属于nitC ,其中nitC在以NaNO2 作为唯一氮源的MO2 培养基上被完全抑制致死。nitA配nitB的营养体亲和性测定结果 ,16个新疆菌株均与非落叶型对照菌株亲和———属于同一营养体亲和群 (VCG) ,未发现新疆有与落叶型对照菌株亲和的棉花黄萎病菌。以互补指数作为营养体亲和性量化评估指标 ,反映出不同突变类型间亲和性差异较大 ;同一VCG内不同菌株间互补指数也有差异 。
Two hundred and twenty five nitrate nonultilizing(nit) mutants were obtained from 19 strains of cotton Verticillium wilt pathogen, V.dahliae, which included sixteen strains collected from different cotton areas in Xinjiang, one defoliating pathotype strain named T 9 from USA,and two strains from other provinces in China. According to their phenotypes on the media containing only one of the three different nitrogen sources respectively,89.33% were identified as nitA, 9.33% were nit B and 1.33% nit C. The mutant nit C was found inhibited into death by the nitrite.The vegetative compatibility test showed that all the 16 strains from Xinjiang.were compatible with VJ, a nondefoliating strain from Shanxi, so they fell into the same vegetative compatibility group(VCG). VCG of defoliating pathotype was not found at present in Xinjiang, Complementary Index(CI) was used as a quantitative index of vegetative compatibility, CI between phenotypes of nit A and nit B in sequence was nit A-nit B>nit B-nit B>nit A-nit A. For example, CI between nitA and nit B (nit A-nit B) were all over50.However, CI between nit B and nit C determined in this paper were all 100.
出处
《新疆农业大学学报》
CAS
2000年第1期7-11,共5页
Journal of Xinjiang Agricultural University
基金
国家"九五"棉花科技攻关资助项目 !(95- 0 0 1- 0 4 - 0 4 )
关键词
大丽轮枝菌
亲和性评估
VCG
棉花黄萎病
cotton
Verticillium dahliae
vegetative compatibility group
assessment
