摘要
用硝酸还原酶缺陷型(Nit)突变体技术,对来自江苏省80年代及1991年不同菌株进行了营养体亲和性研究。80年代的65个大丽轮枝菌菌株经含氯酸钾培养基诱导,表型鉴定获得突变体A型(Nit 1)73株,B型(Nit M)77株,C型27株,其它类型4株。亲和性测定表明,仅一株(VD8)与国外落叶型群相亲和,属同一亲和群,其余菌株为非落叶型群。对1991年的35个菌株诱导获得78个A型突变体,19个B型突变体。有5个菌株与国外落叶型群相一致,呈强亲和性反应,其中JC1B及SY12两菌株获得A、B两型突变体,可成为我国的落叶型的标准菌株。
Over 100 strains of V. dahliae were isolated from cotton, eggplant and several other plants in Jiangsu Province in 1980s and in 1991. Near 300 nitrate nonutilizing (Nit) mutants were recovered from those strains by growing on minimal media with 22. 5g/L Patassium Chlorate. The Nit mutants could be divided into 4 phenotypes by their growth on different supplemental minimal agar media. 151 mutants of NitA (Nitl) and 96 mutants of NitB (NitM) were majority (85.67％) of all Nit mutants. NitC and other phenotypes merely occupied 12.50％ and 3.47％, respectively. Vegetative compatibility was indicated by the development of linear growth of wild type with abundant micosclerotia and/or dense mycelium. Among 58 strains isolated from cotton, eggplant and other plants in 1980s, only one strain VD8, which isolated from Xinkeng Village, Henxing Town, Nanton County, was defoliating strain corresponding to Puhalla's VC group 1; all other strains were classified into non—defoliating VC group. In the contrast, 5 strains isolated from cotton in 1991 were classified into the defoliating VC group, the others were belonged to nondefoliating VC group. Those defoliating strains were located at 4 sites, 2 counties (Nantong and Changshu) where defoliating symptoms were discovered in 1983. The pathogenicity of two strains in deffernt VC groups was evaluated under control condition, the defoliating strain SY12 appeared typical defoliating symptom and showed higher agressiveness than that of the non—defoliating strain DF14. The Nit mutant technique should be used to monitor the distribution of defoliating straing of V. daMiae.
出处
《棉花学报》
CSCD
北大核心
1993年第2期79-86,共8页
Cotton Science
关键词
大丽轮枝菌
营养体亲和性
棉花
Verticillium, Defoliating strains, Nit mutant, Vegetative compatibility
