摘要
背景:研究证实脐带间充质干细胞、番茄红素均有不同程度的抗衰老作用,而关于二者联合抗衰老作用的研究国内外研究甚少,目前基本处于空白状态。目的:观察人脐带间充质干细胞联合番茄红素对D-半乳糖衰老模型小鼠的抗衰老作用。方法:实验选用ICR小鼠,通过颈背部皮下注射D-半乳糖复制亚急性衰老小鼠模型,分为对照组、模型组、干细胞治疗组、番茄红素治疗组和干细胞联合番茄红素治疗组。实验8周后处死,测定各组小鼠血清、肝脏、脑组织中丙二醛含量及超氧化物歧化酶活性及皮肤中羟脯氨酸含量。结果与结论:与对照组相比,模型组小鼠超氧化物歧化酶活性、羟脯氨酸含量降低,丙二醛含量升高,差异有非常显著性意义(P≤0.01)。与3个治疗组相比,模型组超氧化物歧化酶活性、羟脯氨酸含量偏低,丙二醛含量偏高,且差异有显著性意义(P≤0.05),但模型组大脑中丙二醛含量与番茄红素治疗组差异无显著性意义(P>0.05)。3个治疗组相比,干细胞联合番茄红素治疗组中超氧化物歧化酶活性、羟脯氨酸含量较高、丙二醛含量较低,差异有显著性意义(P≤0.05)。提示干细胞、番茄红素具有不同程度的抗衰老作用,且二者联合的抗衰老作用优于单独使用。
BACKGROUND： Studies have demonstrated the anti-aging action of human umbilical cord mesenchymal stem cells or lycopene. However, there are few researches on the anti-aging action of their combination. OBJECTIVE： To investigate the anti-aging effects of human umbilical cord mesenchymal stem cells combined with lycopene on D-galactose-induced aging model in mice. METHODS： Subactute aging model was established in ICR mice by subcutaneous injection of D-gatactose. The experimental mice were divided into control group, model group, stem cell group, lycopene and stem cells＋lycopene group. The mice were sacrificed after 8 weeks. The activity of superoxide dismutase, the content of malondialdehyde in serum, liver, brain and the content of hydroxyproline in skin were determined. RESULTS AND CONCLUSION： Compared with the control group, the activity of superoxide dismutase and thecontent of hydroxyproline were significantly reduced, but the content of malondialdehyde significantly increased in model group mice （P ≤0.01 ）. Compared with three treatment groups, the activity of superoxide dismutase and the content of hydroxyproline were significantly lower, but the content of malondialdehyde significantly greater in the model group （P≤0.05）. However, there was no statistical significance in content of malondialdehyde between model and lycopene groups （P 〉 0.05）. The activity of superoxide dismutase and the content of hydroxyproline were significantly higher, but the content of malondialdehyde significantly lower in stem cells＋lycopene group compared with stem cell or lycopene groups （P ≤ 0.05）. Results suggested that human umbilical cord mesenchymal stem cells and lycopene have anti-aging effects, and the anti-aging effects of the combination of them are better than either of them.
出处
《中国组织工程研究》
CAS
CSCD
2013年第40期7040-7046,共7页
Chinese Journal of Tissue Engineering Research
基金
北京大学航天临床医学院科研基金项目(201110)~~
