摘要
类番茄茄(Solanum lycopersicoides)高抗黄瓜花叶病毒(Cucumber mosaic virus,CMV)。通过4次独立试验,对来自类番茄茄LA2951的渐渗系(Introgression Line,IL)采用摩擦接种法,人工接种CMV亚组Ⅱ进行了筛选,定位了8个抗CMV的QTL,它们分别位于番茄第2、7、8、9、10、11和12染色体上,均可显著降低植株的病情指数,与前人已鉴定的来自多毛番茄(S.habrochaites)LA1777和智利番茄(S.chilense)LA0458的抗CMV位点不同位,为抗CMV新位点。通过对抗CMV的IL初步分析发现,这些IL包含的QTL呈现不完全显性遗传效应,QTL聚合为完全加性效应。
Solanum lycopersicoides showed high resistance to Cucumber mosaic virus （CMV） . An introgression line （IL） population derived from a nightshade related species S. lycopersicoides LA2951 was used to identify the involved loci for resistance to CMV by rub-inoculation in four independent experiments. In total, 8 QTLs for resistance to CMV located on chromosome 2, 7, 8, 9, 10, 11 and 12 were found respectively. Percentage of disease index （PDI） was significantly decreased in these ILs. All QTLs were new loci when compared those identified previously from S. habrochaites LA1777 and S. chilense LA0458. The genetic analysis of these QTLs showed incompletely dominant. The interaction of these QTLs presented complete additive effect. The results provide a prospect for breeding of CMV resistance in tomato.
出处
《园艺学报》
CAS
CSCD
北大核心
2013年第10期1905-1915,共11页
Acta Horticulturae Sinica
基金
公益性行业(农业)科研专项经费项目(201303115)
农业部园艺作物生物学与种质创制重点实验室项目
关键词
番茄
类番茄茄
渐渗系
黄瓜花叶病毒
QTL定位
tomato
Solanum lycopersicoides
introgression line
Cucumber mosaic virus （CMV）
QTL mapping
