摘要
目的探讨兰州地区慢性乙型肝炎病毒感染者血清乙型肝炎病毒大蛋白(HBV-LP)检测的临床意义。方法选择兰州地区慢性乙型肝炎病毒(HBV)感染者1 036例,采用酶联免疫吸附法(ELISA)检测HBV-LP吸光度值,化学发光免疫分析法(CLIA)检测乙型肝炎病毒血清标志物(HBV-M)含量。结果兰州地区1 036例慢性HBV感染者中HBV DNA阳性率为72.01%(746/1036),HBV-LP阳性率为84.36%(874/1036),检出一致率为78.19%。HBV-LP的表达与HBV DNA水平呈正相关关系(r=0.94),差异具有统计学意义(P<0.01);不同HBV-M模式中HBV DNA与HBV-LP的检出率不同,HBsAg和HBeAg同时阳性者HBV DNA与HBV-LP均有较高的阳性率,HBeAg阴性者HBV DNA与HBV-LP的阳性率均较低。结论兰州地区慢性乙型肝炎病毒感染者血清HBV-LP吸光度与HBV DNA拷贝数的对数值有较好的相关性,HBV-LP表达与病毒复制有关,HBV-LP的检测能够反应HBV DNA的复制情况。
Objective To discuss the significance of HBV-LP（hepatitis B virus large protein）detection in patients with HBV chronic infection in Lanzhou.Methods Selected 1036patients with HBV chronic infection,using ELISA to detecting HBV-LP,and CLIA to detecting the assay of HBV-M.Results In 1036 patients,positive rate of HBV DNA is 72.01%,positive rate of HBV-LP is 84.36%,concordance rate of detection is 78.19%,HBV-LP expression and HBV DNA level showed positive correlation（r=0.94）,and the difference between both was statistically significant（P＆lt;0.01）.In HBV M model,HBV DNA and HBV-LP detecting rate is are different;Patients have positive in HBsAg and HBeAg show high positive rate in both HBV DNA and HBV-LP,and HBeAg negative show low positive rate.Conclusion Serum HBV-LP absorbance and log value of HBV DNA copy shows nice correlation in patients with HBV chronic infection in Lanzhou.HBV-LP expression has some relationship with replication of virus,HBV-LP can reflect the HBV DNA replication.
出处
《国际检验医学杂志》
CAS
2013年第20期2667-2668,共2页
International Journal of Laboratory Medicine
