摘要
目的通过对乙型肝炎患者HBV-LP、Pre-S1抗原、HBV-DNA载量和乙型肝炎血清标志物的检测,探讨反映不同血清学类型患者体内病毒复制及抗病毒疗效监测的最佳指标以及分析各指标之间的关系。方法对864例HBV感染者及100例健康体检者血清采用ELISA法定性检测HBV-M;ELISA双抗体夹心法检测HBV-LP、Pre-S1;荧光定量PCR法检测HBV-DNA。结果 HBsAg、HBeAg、HBcAb阳性模式组(大三阳)HBV-LP、Pre-S1及HBVDNA阳性率显著高于其它HBV-M模式组(P<0.05);HBV-LP与HBV-DNA的阳性率无显著差异(P>0.05),PreS1的阳性率明显低于HBV-LP与HBV-DNA的阳性率(P<0.05);HBV-DNA拷贝数与HBV-LP、Pre-S1平均A值具有明显的相关性(相关系数r=0.926),而与HBsAg定量值无明显相关性。结论 HBV-LP比Pre-S1试剂效果大大提高,且大蛋白的检出率与血液中病毒DNA的检出高度符合,对于判断病毒复制具有重要意义,可作为HBeAg及HBV-DNA的补充检测指标。
Objective To investigate the best indicator which is helpful in visualizing vivo viral replication and anti viral efficacy monitoring in examination for different types of serological patients and analysis the relationship between indicators through detections of HBV-LP,Pre-S1 antigen, HBV-DNA and serum markers of hepatitis B in patients in- fected by HBV. Methods 864 cases of HBV infection and 100 cases of healthy persons＂ serums was collected and ser um HBV-M was detected by ELISA method, HBV-LP was detected by ELISA double antibody sandwich assay and HBV-DNA was detected by fluorescence quantitative PCR. Results HBV-LP,Pre-S1 and HBV-DNA positive rate was significantly higher in the group of HBsAg, HBeAg, HBcAb positive mode （HBeAg） than other HBV-M model group （P〈0.05） ; HBV-LP and HBV-DNA positive was no significant difference （P〉0.05） ,Pre-S1 positive rate was signif icantly lower than HBV-LP and HBV-DNA positive rate （P〈0.05）; HBV-DNA copy number of HBV-LP,Pre-S1 av erage a value has a significant correlation （correlation coefficient, r= 0. 926）, while the quantitative value of HBsAg was no significant correlation. Conclusion The effect of HBV-LP reagents are greatly improved than the Pre S1 and large protein detection rate was relevant to blood viral DNA detection. HBV-LP has significant for judging viral replication and can be used as complementary indicators of HBeAg and HBV-DNA.
出处
《中国实验诊断学》
2013年第9期1634-1637,共4页
Chinese Journal of Laboratory Diagnosis
基金
吉林省科技厅重点实验室项目(20122113)
