摘要
对高效液相色谱(HPLC)测定红曲中内酯型Lovastatin的方法进行研究。采用75%乙醇对红曲中的Lovastatin超声提取20min。 离心后用中性氧化铝柱层析吸附上清液中的色素。一定条件下Lovastatin不被吸附而随着洗脱液流出。经过前处理后的样品以甲醇：0.1%磷酸（V/V）75：25为流动相在1ml/min流速下用反相高效液相色谱分离去除残余色素及其它杂质干扰，内酯型Lovastatin用紫外检测器在238nm波长下检测。在本实验条件下7次重复测定加标样品的回收率为98%±5% ，标准样品7次重复测定的变异系数为2.63% 。样品中内酯型Lovastatin的最低检测下限为4μg。利用本实验条件成功测定了两种红曲发酵制品中内酯型Lovastatin的含量。
s: High performance liquid chromatography (HPLC) quantitative determination for Lovastatin in Monascus was studied. 75% ethanol for Lovastatin ultrasonic extraction from Monoscus was used . Extraction was continued for 20 minutes. After centrifugation, the supernatant pigment was adsorbed on a neutral aluminum oxide column. Under the particular condition Lovastatin could not be adsorbed and eluted with the effluent. After pretreatment, crude Lovastatin sample was treated by a reverse phase HPLC (RP-HPLC) column for further purification to remove the remaining pigment and other impurity. The motive phase of RP-HPLC was as follows : Methanol :0.1%, phosphate (V/V)= 75:25, and the flow rate 1ml/min. Purified Lovastatin was measured by an ultraviolet detector under 238 nm . According to 7 repeated determinations of Lovastatin sample plus standard , under the experiment condition, the recovery rate was 98%±5% with variance 2.63% . The minimum allowable detection of Lovastatin in a sample was 4 μg . Using the experiment condition ,the Lovastatin contents in two Monascus fermentation products were successfully determined.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2000年第12期100-102,共3页
Food Science
基金
中国发酵工业协会特种功能发酵制品专业委员会资助
