摘要
以提取过药用成分的杜仲叶渣为原料,提取杜仲胶。研究了分别用碱水解和酶解预处理杜仲叶渣,然后用溶剂提取杜仲胶,以及直接用溶剂提取杜仲胶的工艺。实验结果表明最佳工艺条件为:杜仲叶渣直接用正己烷作溶剂提取杜仲胶,原料与溶剂比例为1∶20(g∶mL),机械搅拌并加热回流浸提2 h,趁热过滤,滤液放入冰箱在-20℃条件下冷却析晶1 h,真空抽滤后自然干燥得到浅绿色杜仲胶产物,提取得率为4.42%。用丙酮多次精制得到白色的杜仲胶。对白色杜仲胶产物做红外光谱测定,确定为反式1,4-聚异戊二烯(TPI)。
Residue of Eucommia ulmoides leaves, after being processed for pharmaceutical ingredients, was used to extract Eucommia gum. Both of solvent extraction of Eucomraia gum, with pretreatment of E. ulmoides leaf residue by aJkali hydrolysis and enzymatic hydrolysis separately, and direct extraction were studied. The optimum process condition was that： E. ulmoides leaf residue was mixed with n-hexane by a material to solvent ratio of 1 ： 20 （ g ： mL）, reflux extraction was applied at a high temperature for 2 h. Filtrate was put in freezer for 1 h at - 20 ℃ for cooling crystallization, and then vacuum filtered and naturally dried to obtain the product of light green Eucommia gum. The yield was 4.42%. White Eucommia gum was obtained after multiple times of refining by acetone. The infrared spectrum of white Eucommia gum was measured, It was identified as trans-1,4-polvisoorene （TPI）.
出处
《生物质化学工程》
CAS
2013年第5期35-39,共5页
Biomass Chemical Engineering
基金
北京林业大学大学生创新创业训练计划(121105)
农业科技成果转化基金项目(2009GB23600509)
关键词
杜仲叶
杜仲胶
提取
Eucommia ulmoides leaf
Eucommia gum
extraction
