摘要
实验用12头黑白花奶牛(3-9岁),PGF_(2α)作同步发情处理后,周期第10-12天,总剂量36mg的FSH-P按递减方式每日肌肉注射2次,间隔12小时,共注射4天,在第3天加注PGF_(2α) 35mg,进行超数排卵处理。12头供体牛共得胚135枚,可用胚数118枚,为总胚数的87.47％。平均每头得胚11.25枚,可用胚9.8枚。其中有3头供体共得胚76枚(分别为24、25和27枚),可用胚69枚,平均每头可别胚高达23枚。胚胎分别移植给20头同步发情处理的黑白花奶牛和20头黄牛受体(有3头为自然发情),海头移植1枚胚胎。结果,奶牛受孕率为35％(受孕7头,产犊7头);黄牛受孕率为40％(受孕8头,流产2头,产犊6头)。奶牛和黄牛总受孕率为37.5％。
12 donors (3 - 9 years old dairy cows) were superovulated with FSH-p twice daily in the dose of 36 mg for four days in 10-12th days of cycle after synchronized with PGF2a. At the third day of supcrovulation, additional 35 mg PGF2a were administrated intramuscularly. A total of 135 embryos were recovered 6-7 days after insermination. Of these embryos, 87.47% (118/135) showed good quality (average 9.8 embryos per donor). Particularly, we got 76 embryos from 3 donors (24, 26 and 27 respectively) and 69 of 76 were good quality (23 embryos per donor) . After transferred to synchronized recipients (20 dairy cows and 20 cattle) with total 40 embryos. 7 dairy cows (35%) and 8 cattle (40%) were pregnant. The average pregnant rate were 37.5%. A total of ]3 heifers were delivered to birth from 7 dairy cow and 6 cattle in this experiment.
基金
云南省科委农医处资助项目
关键词
奶牛
黄牛
胚胎
移植
Dairy cow, Cattle, Superovulation, Embryo transfer
