摘要
用Ｂａｌｂ／ｃ雄小鼠的脾细胞和皮肤组织对同系雌小鼠脾脏作一次直接免疫和５次腹腔加强免疫，雌小鼠产生良好的免疫应答。其抗血清用未经免疫的雌小鼠脾细胞吸收后，经细胞毒性法检测，Ｈ－Ｙ抗血清的效价可达１：１０２４；选择免疫应答最好的２只雌小鼠，取其脾细胞与ＮＳ－１骨髓瘤细胞进行融合，融合率１００％，经３次有限稀释克隆后，取４只阳性最强的杂交瘤细胞制备腹水。取其中一株的腹水采用胚胎细胞毒法进行特异性鉴定，结果其对２０个正常胚胎中的７个有杀伤溶解现象。４株细胞系分泌的抗体（腹水）以精子细胞毒法测定其效价分别为１：５１２、１：５１２、１：２５６和１：１２８。
After Balb/c female mice were primarily immunized by intra-splenic injection of Balb/c male splenocytes,and then by intraperitoneal injection of male minced skin tissue for five times,they mounted a high immune response.The sera collected from immunized female mice were absorbed by splenocytes from non-immunized femalemice,and were subjected to sperm cytotoxicity test giving a titer of 1024.NS-l cells and splenocytes from immunized mice were fused in 50%PEG.The fusion rate was100%.After being loned three times by limiting dilution,bur clones of hybridomas that could secret monoclonal antibodies were obtained.The specificity of monoclonalantibodies were determined by embryo cytotoxicity test.The titers of monoclonal antibodies detected with the method described above were 1:512,1:512.1:256 and1:128 respectively
出处
《华南农业大学学报》
CAS
CSCD
1996年第2期38-43,共6页
Journal of South China Agricultural University
关键词
H-Y
抗原
单克隆抗体
间接免疫荧光
胚胎细胞毒
H-Y antigen,monoclonal antibodies,indirect immunofluorescence
embryo cytotoxicity test present address:Dept of immunology,The Kunming institute of Zoology,Academid'Sinica,Kunming,650223
