摘要
目的:建立小鼠肝癌休眠模型并验证休眠细胞的存在。方法:30只昆明种小鼠均右侧腋下接种1×106个H22腹水型肝癌细胞,常规喂养2周,第15天选择表皮肿瘤直径>0.5cm的小鼠,进行姑息性手术,将肉眼可见肿瘤组织全部切除。手术后的小鼠再常规喂养8周,如接种处未见肿瘤组织生长,即认为肝癌小鼠休眠模型建立成功(按照平均寿命折算,小鼠生存期8周,即相当于人类寿命5年)。其后模型小鼠分为复发对照组和复发实验组,复发对照组小鼠常规喂养;复发实验组模型小鼠给予连续外伤刺激。6周后处死全部模型小鼠,接种部位取材,组织固定和HE染色观察肿瘤再生成情况确定休眠细胞的存在。结果:肝癌细胞接种于小鼠并行手术后,有20%小鼠成瘤,80%处于休眠状态。复发实验组给予连续外伤刺激6周后,100%小鼠成瘤,而复发对照组只有8.3%,两组差异有统计学意义,P=0.000。结论:本方法成功建立了小鼠肝癌休眠模型,并用外伤刺激引起肝癌休眠细胞增殖,验证了休眠肿瘤细胞在体内的存在。
OBJECTIVE：To establish a mouse hepatoma dormancy model and provide the proof for the existence of dormant tumor cells.METHODS：The hepatoma H22cells（1×106 cells）were injected into a subcutaneous of right armpit of each Kunming（KM）mice.Then,palliative surgery was performed in the mice which the diameter of tumor was large than 0.5cm at the 15th day after inoculation.After another 8 weeks the mice without recurrence were regarded as the successful hepatoma dormancy model.Those hepatoma dormancy mice were randomly divided into two groups,the recurrence group and the control group.Then,cut off the tail of mice in the recurrence group 2mm every 4days and repeated for 5times.The mice in both groups were fed normally during the same time.During the time,if the tumor recurrence in mouse the tumor size was measured.The tumor cells were assessed by histopathology when the mice in two groups were sacrificed in 6 weeks.RESULTS：The results showed that the success rate of hepatoma dormancy model was 80%.The recurrence rate of hepatoma in recurrence group was 100%,and in control group was 8.3%respectively.The recurrence rate of recurrence group was significantly higher than that in control group（P=0.000）.CONCLUSION：The mouse hepatoma dormancy model was successfully established by palliative surgery,and the recurrence of hepatoma could be induced by cutting off the tail of mice repeatedly.
出处
《中华肿瘤防治杂志》
CAS
北大核心
2013年第19期1484-1486,共3页
Chinese Journal of Cancer Prevention and Treatment
基金
广东省中医药局建设中医药强省科研课题(2010429)
关键词
肝肿瘤
休眠
疾病模型
动物
小鼠
liver neoplasms dormancy disease models animal mice
