摘要
目的:探讨三氧化二砷(AS2O3)对小鼠肺癌移植瘤的抑制作用,进一步分析其抗血管生成的可能机制。方法:建立Lewis肺癌小鼠移植瘤模型,60只小鼠随机分成阴性对照组、阳性对照组和AS2O3治疗组3组(每组20只),应用AS2O3治疗后,观察肿瘤生长情况计算抑瘤率,免疫组化法检测肿瘤组织内血管内皮生长因子(VEGF)和表皮生长因子受体(EGFR)的表达。结果:AS2O3组、阳性对照组、阴性对照组的瘤质量分别为(1 420±60)、(1 500±80)和(2 420±190)mg;AS2O3组和阳性对照组的瘤质量较阴性对照组明显减轻,P<0.05;AS2O3组的抑瘤率(52%)较阳性对照组(54%)差异无统计学意义,P>0.05。AS2O3治疗后VEGF、EGFR阳性细胞比例分别为(63.10±5.32)%、(68.33±5.99)%,较阴性对照组〔(76.33±10.3)%、(80.50±10.21)%〕降低,且差异有统计学意义,P<0.05。结论:AS2O3对小鼠肺癌移植瘤有显著的抑制作用;下调VEGF和EGFR的表达是AS2O3抗肿瘤血管生成的可能机制。
OBJECTIVE： To evaluate the anti-angiogenesis effects of arsenic trioxide on transplanted lung carcinoma in mice. METHODS： Lewis lung carcinoma ceils （LLC cells） were transplanted subcutaneously to C57BL/6 mice to gener ate tumors. Sixty mice were randomly divided into3 groups （n=20） ：negative control group,positive control group andar senic trioxide treatment group. The tumor weight of the mice was measured respectively during the therapy. The tumor growth inhibition rates of each group were calculated. The expressions of VEGF and EGFR in the lung cancer tissue were examined by immunohistochemical method. RESULTS：The tumor weight of arsenic trioxide treatment group, positive control group and negative control group were （1 420±60）,（1 500±80） and （2 420± 190） mg respectively. Compared with negative control group, the tumor weight of arsenic trioxide treatment group and positive control group were significantly lower than that of the negative control group （P〈0.05）. The growth inhibiting rates of tumor in arsenic trioxide treat ment group and positive control group were no significant different （P〉 0. 05）. The expression of VEC-F （63. 10± 5.32）% and EGFR （68. 33 ±5. 99）% in arsenic trioxide treatment group were significantly less than those in negative control group [（76.33±10.3）%,（80.50±10. 21）%,P〈0.05].CONCLUSION： Arsenic trioxide has strong inhibition effect on tumor growth;This effect was probably mediated by downregulating the expression of VEGF and EGFR.
出处
《中华肿瘤防治杂志》
CAS
北大核心
2012年第21期1625-1627,1639,共4页
Chinese Journal of Cancer Prevention and Treatment
关键词
肺肿瘤
砷剂
疾病模型
动物
肿瘤移植
lung neoplasms
arsenical
disease models,animal
neoplasms transplantation
