摘要
探讨冻干处理对聚合人胎盘血红蛋白质量的影响。设计适宜的实验条件对聚合人胎盘血红蛋白(PolyPHb)溶液进行冻干处理后,检测冻干前后的血红蛋白浓度、高铁血红蛋白(MetHb)含量、氧结合量、游离三价铁离子(Fe3+)含量、pH值、紫外线(UV)光谱扫描、平均分子量及其分布、圆二色谱扫描、十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)、氧饱和曲线等指标的变化以及冻干品的外观、残余含水量、复溶时间等是否符合要求。结果表明:5批冻干处理样品的各项检测指标在冻干前后均无显著变化。这说明冻干处理对PolyPHb溶液理化性质及生物活性无显著影响,因此可以将PolyPHb溶液按本工艺进行冻干保存。
The present study was aimed to investigate the influence of freeze drying on the quality of polymerized hu- man placenta hemoglobin （PolyPHb）. The PolyPHb solution was freeze-drying under suitable conditions. Hemoglo- bin concentration, methemoglobin （MetHb） content, UV spectrum, Fe3- content, oxygen-carrying capacity, pH, the average molecular weight and its distribution, circular dichroism, oxygen equilibrium curve and other indicators were measured before and after freeze drying. The appearance, residual water content, rehydration time of the lyoph- iiized product were also evaluated. The results showed that there was no significant difference on all the indicators measured above, which indicated that freeze-drying process had no effect on the physical and chemical properties of PolyPHb, as well as on its biological activity. Therefore, the properties of PolyPHb were stable during this freeze- drying process and could be preserved after such freeze-drying process.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
2013年第5期1052-1057,共6页
Journal of Biomedical Engineering
基金
863项目资助(2012AA021903)
关键词
聚合人胎盘血红蛋白
冻干
高铁血红蛋白
Polymerized human placenta hemoglobin solutions （PolyPHb）
Freeze drying
Methemoglobin （MetHb）
