摘要
利用聚乙二醇(PEG)/硫酸铵双水相系统分离纯化盐析得到了血红蛋白(HB)样品,考察了PEG相对分子质量、PEG质量分数、(NH4)2SO4质量分数、pH、温度等因素对HB分离的影响。结果表明PEG双水相系统的最佳分离条件为:wPEG400=20%,w(NH4)2SO4=20%,pH=7,温度为30℃。在此条件下HB分配系数可达到65,回收率可以达到89.5%,纯度达到80.5%。
The aqueous two-phase system of PEG/（NH_4）_2SO_4,was used to purify hemoglobin（HB） in the initial salting out sample.The research investigated some parameters such as molecular weight of PEG,mass fraction of PEG and（NH_4）_2SO_4,pH,temperature which will influence partition ratio of hemoglobin.The results show that the best separation condition： w_（PEG400）=20%,w_（（NH_4）_2SO_4）=20%,pH=7,temperature 20 ℃.The highest partition ratio can reach up to 65,the maximum value of extractive rate was 89.5%,and its purity was 80.5%.
出处
《华东理工大学学报（自然科学版）》
CAS
CSCD
北大核心
2010年第5期685-689,共5页
Journal of East China University of Science and Technology
基金
上海市科委应用技术开发项目资助
