摘要
试验比较四种防冻剂对小鼠核泡期(germinal vesicle stage)无卵丘细胞的卵母细胞(下称裸卵),在各种不同条件下冷冻-解冻的影响,并用乙二醇为防冻剂,冷冻-解冻后,形态正常的裸卵1137枚,经体外培养排出第一极体、发育达中期Ⅱ的成熟率为43.2％(491/1137)。用体外获能的小鼠附睾尾精子进行体外受精的受精率为25.4％(31/122)。继续培养进一步发育至2-细胞期的发育率为17.3％(55/318)。对照组(新鲜裸卵)的成熟率为48.6％(158/325),受精率为26.4％(388/144)。两者无显著差异(P<0.05)。
Mouse oocytes at the germinal vesicle stage without cumulus cells were frozen at-196℃. Ethylene glycol was used as cryoprotectant. Oocytes were cooled at 1℃ per min from room temperature to -5℃ where thin plastic tubings were seeded and held for 10 min. The oocytes were then cooled at 0.3℃ per min to-40℃ and plunged directly into liquid nitrogen. Plastic tubings containing oocytes were thawed in a 37℃ water both for 10 min and ethylene glycol was removed with PBS. Oocytes were washed two timesand placed in TALP medium supplemented with 20% fetal bovine serum. The proportion of the oocytes exhibiting primary polar body formation in vitro and the IVE rate were 43.2% and 25.4% for the frozen-thawed oocytes and 48.6% and 26.4% for the nonfrozen control, respectively. Of 318 frozen-thawel oocytes that were fertilized in vitro, 55(17.3%) developed to 2-cell stage embryos.
出处
《动物学报》
SCIE
CAS
CSCD
1991年第2期203-209,共7页
ACTA ZOOLOGICA SINICA
关键词
小鼠
卵母细胞
冷冻
解冻
发育
Mice, Oocyte, Cryoprotectant, Frozen-thawed.
