摘要
目的观察Dickkophl（DKKl）在胶原诱导性关节炎（CIA）大鼠外周血中的水平，探讨其与关节炎症的关系。方法建立CIA大鼠关节炎模型，分为模型组、对照组，采用足爪容积、关节炎指数（AI）评价关节炎症；酶联免疫吸附试验（ELISA）法检测血清中DKK1、骨保护素和肿瘤坏死因子（TNF）-α水平；魏氏法检测红细胞沉降率（ESR），免疫比浊法检测C反应蛋白（CRP）。采用t检验、方差分析、秩和检验和相关分析进行统计学处理。结果模型组大鼠足爪容积大于对照组（模型组：2．01±0．21，对照组：1．55±0．11），差异有统计学意义（t=77．139，P=O．000）；模型组大鼠在造模后每周AI值逐渐增加（分别为7．20±0．25，10．64±2．18，12．81±1．82），差异有统计学意义（F=17．736，P〈0．05）；模型组在造模后1、2、3周血清DKK1水平分别为（60±15），（73±16），（82±20）pg／ml，明显高于对照组[（20±9）pg／ml，F=45．866，P=O．000]；模型组在造模后1、2、3周血清TNF-α分别为（221±102），（300±87），（344±70）pg／ml，明显高于对照组[（101±38）pg/ml，F=27．689，P=0．000]；模型组在造模后1、2、3周血清骨保护素水平分别为（240±168），（130±61），（71±37）pg／ml，明显低于对照组[（450±147）pg／mt]，差异有统计学意义（F=255．29，P：0．000）。相关性分析显示模型组大鼠血清DKKI与AI、TNF-α呈正相关（r值分别为0．428、0．513，P〈0．05）。结论CIA大鼠血清DKK1水平可能与炎症相关，或许是通过TNF-α进行调控的。
Objective To observe the peripheral blood levels of Dickkopf-1 （DKK1） in collagen induced arthritis （CIA） rats, and to explore the relationship with joint inflammation. Methods CIA rats were randomly divided into 3 model groups and 3 control groups, joint inflammation were assessed by paw volume and arthritis index （AI）. Serum levels of DKK1, osteoprotegerin （OPG） and tumor necrosis factor-alpha （TNF-α） were measured by enzyme linked immunosorbent assay （ELISA）. Erythrocyte sedimentation rate （ESR） was detected by Westergren method, while C-reactive protein （CRP） was tested by immunoturbidi- metry. T test, analysis of variance, rank sum test, and correlation analysis were used for statistic analysis. Results Paw volume was significantly enlarged in the model groups （2.01±0.21 vs 1.55±0.11, t=77.139, P=-0.000）. The AI score of the model groups was gradually increased weekly, the difference was statistically significant （7.20±0.25, 10.64±2.18, 12.81±1.82, respectively; F=17.736, P〈0.05）. After modeling for 1, 2, 3 weeks, the serum levels of DKK1 in the models were （60±15）, （73±16）, 82±20） pg/ml respectively, which were signi-ficantly higher than those of the controls （20±9） pg/ml （F=45.866, P=0.000）, TNF-α in the models were （221±102）, （300±87）, （344±87） pg/ml respectively, which were significantly higher than controls （101 ±38） pg/ml （F=27.689, P=0.000）, the ESR and CRP in the models were also significantly higher than those of the controls, while OPG in the models were （240±168）, （130±61）, （71±37） pg/ml respectively, which were significantly lower than the controls （450±147） pg/ml, the differences of above parameters between the two groups were all statistically significant （F=255.29, P=-0.000）. Correlation analysis showed that the serum levels of DKK1 in the model group and AI, TNF-α were positively correlated, the correlation coefficients were 0.428 and 0.513, respectively, the difference was statistically significant （P〈 0.05）. Conclusion Serum levels of DKK1 in the CIA rats may be associated with inflammation, which may be regulated through TNF-α.
出处
《中华风湿病学杂志》
CAS
CSCD
北大核心
2013年第10期685-688,共4页
Chinese Journal of Rheumatology
基金
上海市卫生局中医药科研基金（2012QL043A）
上海市中医药事业发展三年行动计划（ZYSNXD-CCZDYJ054）
上海市长宁区卫生局科研课题（20114Q03001）
