摘要
目的探讨瑞舒伐他汀对同型半胱氨酸（Hcy）损伤的人脐静脉内皮细胞（HUVEC）超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量的影响。方法原代培养HUVEC，2—3代进行实验，用一定浓度Hcy损伤HU—VEC建立细胞损伤模型，然后瑞舒伐他汀干预研究，采用黄嘌呤氧化酶法测定SOD的活性，硝酸还原酶法检测细胞上清的一氧化氮（NO）含量，硫代巴比妥酸法检测MDA的水平；Real—timePCR检测SODlmRNA表达的变化。结果Hcy刺激24hSODlmRNA表达量、NO、SOD浓度较空白对照组显著降低，MDA含量较空白对照组显著升高（P〈0．05）；瑞舒伐他汀预处理2h后再加入等浓度Hcy，刺激24h后SODlmRNA表达量、NO、SOD浓度显著升高，MDA含量显著下降（P〈0．05）。结论瑞舒伐他汀可通过改善SOD活性和减低MDA水平发挥抗氧化作用，且这种作用独立于它的降脂作用。
Aim To investigate the effects of rosuvastatin on superoxide dismutase （SOD） and malondialdehyde （MDA） in human umbilical vein endothelial cells（HUVEC） injured by hnmocysteine （Hcy）. Methods The HU- VEC were primary cultured. HUVEC from the second to third generations were stimulated with different concentrations of Hcy and model of cell injury was set up. Then the HUVEC were pretreated with rosuvastatin in different concentration. Real-time PCR was used to detect the expression of SOD1 mRNA. Activity of SOD was detected by xanthine oxidase tech- nique. The content of MDA was determined by TBA method. The level of nitric oxide （NO） in culture cell supernate of HUVEC was measured by nitrate reductase method using NO assay kit. Results Compared with control group, the ex- pression level of SOD1 mRNA and the content of NO and SOD was significantly decreased, the content of MDA was signifi- cantly increased after 24 hours in Hcy group （ P 〈 0. 05 ）, while pretreated with rosuvastatin for 2 hours, the expression lev- el of SOD1 mRNA and the content of NO and SOD was significantly increased, the level of MDA was significantly decreased （P 〈 0. 05）. Conclusion Rosuvastatin shows its anti-oxidative effect through improving SOD activity and reducing the level of MDA in HUVEC injured by Hcy, and the effect is independent on its lipid-lowering effect.
出处
《中国动脉硬化杂志》
CAS
CSCD
北大核心
2013年第9期802-806,共5页
Chinese Journal of Arteriosclerosis
