摘要
目的:研究艳山姜挥发油对氧化低密度脂蛋白(ox-LDL)诱导人脐静脉内皮细胞损伤的保护作用及一氧化氮合酶-一氧化氮(NOS-NO)的影响。方法:体外传代培养人脐静脉内皮细胞,给予0.1μg·mL-1和0.01μg·mL-1艳山姜挥发油孵育24h后,加入100μg·mL-1 ox-LDL作用24h诱导人脐静脉内皮细胞损伤模型,倒置相差显微镜及吉姆萨染色观察细胞形态学变化,比色法分析细胞培养液NO的含量与细胞裂解液NOS分型活力。结果:Ox-LDL与人脐静脉内皮细胞作用24h后,细胞胞膜皱缩,细胞数目减少,tNOS与eNOS活力显著降低,培养液NO含量降低。艳山姜挥发油显著改善细胞形态学变化,提高细胞tNOS与eNOS活力及培养液中NO含量。模型组与艳山姜挥发油组均对iNOS未见显著影响。结论:艳山姜挥发油对ox-LDL诱导人脐静脉内皮细胞损伤具有保护作用,其保护作用与改善eNOS活力,提高NO水平相关。
OBJECTIVE To investigate the protective effects of essential oil from Fructus Apliniae zerumbet （EOFAZ） on human umbilical vein endothelial cells （HUVECs） injury induced by oxidized low density lipoprotein （ox-LDL）, and explore the ameliorating effects on NOS-NO system. METHODS The in vitro cultured HUVECs were pre-incubated with 0. 1 and 0. 01 μg·mL^-1 EOFAZ 24 h, and then exposure to ox-LDL 24 h. The cyto-morphological change was observed by inverted phase contrast microscope and Giemsa staining assay. The NO content in medium and NOS subtype activities in cell lysis were assayed by colorimetric methods according to the instruction of commercial kits. RESULTS After exposuring to ox-LDL for 24 h, the numbers and morphology of HUVECs were significantly changed by the inverted phase contrast microscope and Giemsa staining compared with control group. The NO contents in medium were significantly decrease compared with control group, the tNOS and eNOS activities of cellular lysis as well. Pre^treated with EOFAZ which ameliorated the cytomorphology and in- creased the number of HUVECs, significantly enhanced the activities of tNOS and eNOS of cell lysis, and the NO contents in medium. However, there were no any significant changes of iNOS activity whether in model group or pre-treated with EOFAZ. CONCLUSION EOFAZ can ameliorate the HUVECs injury induced by ox-LDL, and the mechanism maybe related to enhance- ment of eNOS activity of cells and promotion of promote NO secretion.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2012年第24期1937-1940,共4页
Chinese Journal of Hospital Pharmacy
基金
国家自然科学基金(编号:30960531)
贵州省优秀青年科技人才培养计划(黔科合人字[2009]28号)
教育部科学技术研究重点项目(编号:211166)
贵州省省长资金(中药专项)(黔省专合字(2010)153号)
贵州省中药现代化项目(黔科中药字[2012]551号)
贵阳市现代药业计划项目(筑科合同[2011204]17号)
关键词
艳山姜挥发油
人脐静脉内皮细胞
氧化低密度脂蛋白
一氧化氮合酶
一氧化氮
essential oil from Fructus Apliniae zerumbet （EOFAZ）
human umbilical vein endothelial cells （HUVECs）
oxi- dized low density lipoprotein （ox-LDL）
nitric oxide synthase （NOS）
nitric oxide （NO）
