摘要
目的:研究安息香提取物对损伤内皮细胞中炎性介质乳酸脱氢酶(LDH)、肿瘤坏死因子(TNF-α)及白细胞介素-8(IL-8)活性的调节作用,探究安息香治疗动脉粥样硬化的作用机制。方法:应用补体蛋白纯品于体外在人脐静脉内皮细胞表面组装亚溶破剂量的C5b-9,建立内皮损伤模型,设立安息香提取物高、中、低剂量组,苯甲酸高、中、低剂量组,模型对照组及空白对照组,分别用不同浓度的安息香提取物及苯甲酸标准品处理细胞,应用紫外分光光度法及Elisa试剂盒测定细胞培养上清液中LDH、TNF-α及IL-8浓度。结果:安息香提取物高、中、低剂量组与模型对照组比较,LDH及IL-8分泌水平差异均有统计学意义(P<0.01);安息香提取物高剂量组中LDH及IL-8的分泌水平与空白对照组水平接近,差异无统计学意义(P>0.05);与模型对照组比较,安息香提取物中剂量组TNF-α分泌水平显著降低(P<0.01)。结论:安息香可能通过降低炎性介质LDH、TNF-α及IL-8活性,从而修复内皮细胞损伤。
Objective： To research the regulation of Benzoin extract for inflammatory mediators LDH, TNF-α and IL-8 in endothelial ceils and explore the mechanism of Benzoin treatment of atherosclerosis. Methods： Sublysis dose of C5b-9 was assembled on human umbilical vein endothelial cell surface in vitro by pure complement proteins assembly, and endothelial injury model was established. Groups were built; ceils were cultured in different concentration drugs. Then the concentration of LDH, TNF-α and IL-8 in cell culture supernatant was measured by UV and Elisa kits. Results： LDH and IL-8 activity were reduced in extract high-dose group significantly vs model control group （P〈0.01）, TNF-α levels was reduced in medium-dose groups vs model control group （P〈0.01）. Conclusion： Benzoin may repair endothelial injury cells for therapy atherosclerosis by reducing the activity of inflammatory mediators LDH, TNF-α and IL-8.
出处
《中医药导报》
2014年第1期6-7,10,共3页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
国家青年科学基金项目(络衡方调节补体攻膜复合物C5b-9生物活性表达的物质基础研究
项目编号:30901955)
