摘要
建立桂枝药材HPLC指纹图谱,为桂枝药材的真伪鉴别及质量控制提供新方法。采用Agilent Eclipse XDB-C18色谱柱,以乙腈-水为流动相进行梯度洗脱,流速为0.8 mL/min,柱温为25℃,检测波长为285 nm,进样量5μL,进行了13批次桂枝药材的分析。该分析方法具有良好的精密度、重现性和稳定性,13批桂枝指纹图谱有13个共有峰,4个特征峰,相似度分析表明,广西、广东产桂枝药材质量差异不大。桂枝HPLC指纹图谱有望成为桂枝药材真伪鉴别及质量控制的有力工具。
We constructed high performance liquid chromatography （HPLC） fingerprint for Ramulus Cinnamomi to provide a new method for its quality evaluation and identification.We analyzed thirteen batches of Ramulus Cinnamomi with C18 column （Agilent Eclipse XDB-C18 column）,gradient elution solvent system including water and acetonitrile,flow rate of 0.8 mL/min,column temperature of 25℃ and detection wavelength of 285 nm.The method had better precision,repetition and stability.Thirteen shared peaks and four specific peaks exist in the thirteen batches of fingerprints.Similarity analysis shows that the quality of Guangdong and Guangxi samples has slight difference.HPLC fingerprinting will be a potential identification and quality evaluation tool for Ramulus Cinnamomi.
出处
《山东科学》
CAS
2013年第5期47-51,共5页
Shandong Science
基金
国家"重大新药创制"科技重大专项(2011ZX09201-201-26)
山东省科学院科技发展基金项目(科基合字(2012)第15号)
山东省大型分析仪器升级改造项目(2012SJGZ01)
山东省科技攻关计划(2011GSF11908)
关键词
桂枝
HPLC
指纹图谱
Ramulus Cinnamomi
HPLC
fingerprint
