摘要
本研究旨在探讨阿魏酸钠 (SF)对实验性晶体氧化损伤的抑制作用 .实验设对照组 ,Fenton模型组 ,吡诺克辛 (PS)组和 SF组并分别配制培养液 .将新西兰白兔双眼随机分组后无菌操作摘出眼球 ,立即在手术显微镜下取出晶体 ,称重后将晶体分别放入各组培养液中置二氧化碳培养箱温育 2 4 h.将温育后的晶体冰浴下制成匀浆 .取匀浆测定晶体可溶性蛋白 (SP) ,谷胱甘肽 (GSH) ,维生素 C(Vit C)和丙二醛 (MDA)含量及超氧化物歧化酶 (SOD) ,谷胱甘肽过氧化物酶 (GSH- Px)的活性 .结果表明SF组的晶体 SP,SOD,GSH- Px,GSH,Vit C的含量及活性均显著高于 Fenton组和 PS组 ,而脂质过氧化终末产物 MDA水平显著低于 Fenton组和PS组 .结果提示 SF对实验性晶体氧化损伤有很强的抑制作用 ,并明显优于
To investigate the inhibition of experimental oxidative damages of lens by sodium ferulate, 20 New Zealand rabbits (40 eyes) were divided randomly into four groups: control group, Fenton group (Fenton), pirenoxine sodium group (PS), and sodium ferulate group (SF). Eyeballs were extracted under the condition of sterility immediately. Lenses were drawn, bathed in different media of above groups and incubated in CO 2 incubator for 24 h. Soluble protein (SP), superoxide dismutase (SOD), glutathione peroxidase (GSH Px), glutathione (GSH), vitamin C (Vit C) and malondialdehyde (MDA) in homogenized lenses were measured. The results showed that SP, SOD, GSH Px, GSH, Vit C of SF group were higher than those in Fenton and PS groups; MDA of SF group was lower than that in Fenton and PS groups. The results indicate that SF inhibits experimental oxidative damages of lens, and it is more effective than PS. The study provides a scientific basis to prevent and treat cataract by using SF as a drug.
出处
《中国药理学与毒理学杂志》
CAS
CSCD
北大核心
2000年第6期430-433,共4页
Chinese Journal of Pharmacology and Toxicology
基金
国家中医药管理局科研基金资助重点课题(97Z034)
关键词
阿魏酸钠
白内障
sodium ferulate
cataract
