期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

埃可病毒1型河南分离株全基因组测序及序列分析

Complete genome sequencing analysis of an Echovirus 1 (2140/Henan/2010) strain isolated in Henan province of China
原文传递
导出
摘要 目的了解河南省与手足口病相关的埃可病毒1型(echovirus 1,E1)河南分离株2140/Henan/2010的基因组特征,对其进行全基因测序,并与其他肠道病毒基因进行同源性比较和进化分析。方法采用RT-PCR扩增全基因,采用SeqMan将所测的全部序列进行拼接,使用MegAlign进行同源性比较,MEGA4进行进化分析。结果 2140/Henan/2010基因组全长7 325bp。与原型株Farouk比较,编码区氨基酸同源性为97.6%,核苷酸同源性为80.7%,其中P1核苷酸差异为21.2%,VP1核苷酸差异为16.5%,P2、P3核苷酸差异分别为19.9%、22.4%。进化分析发现,2140/Henan/2010和E1/Farouk仅在P1区和VP1区形成同一分枝,2140/Henan/2010和E1/Farouk在P1和VP1区、P2区、P3区分别与E4、E30、E12亲缘关系最相近。结论与原型株相比,2140/Henan/2010株编码区氨基酸序列差异很小,核苷酸变异较大,核苷酸变异多为无义突变,不影响氨基酸序列的变化;推测E1在P1以外的多个基因组区段分别与其他血清型肠道病毒发生重组。 Objective To sequence the whole genome of Echovirus 1 （2140/Henan/2010 strain） and compare with other enteroviruses. Methods The whole genome was sequenced by RT-PCR; sequence assembly, nu- cleotide and amino acid sequence analysis, and phylogenetic analysis were done by using software SeqMan, MegAlign and MEGA4. Results The genome of 2140/Henan/2010 strain was 7 325 nucleotide long that shared 80.7% and 97.6%homology in nucleotides and deducted amino acids, respectively with the prototype strain E1/Farouk. 2140/Henan/2010 and E1/Farouk are clustered into the same evolutionary group and both strains are most closely related to E1 are E4, E30, and El2 in the Pl, P2, and P3 regions, respective- ly. Conclusions Comparing with the prototype strain, the coding region of 2140/Henan/2010 strain has a si- lent mutation but most nucleotide variations do not bring changes in the corresponding amino acids. Different gene fragments other than P1 region of E1 may recombine with different enterovirus serotypes.
作者 卫海燕 穆玉姣 许玉玲 黄学勇 马宏 陈豪敏 许汴利
机构地区 河南省疾病预防控制中心传染病预防控制所
出处 《中国病毒病杂志》 CAS 2013年第5期377-381,共5页 Chinese Journal of Viral Diseases
基金 河南省医学科技攻关计划重大项目(201001015)
关键词 手足口病 埃可病毒1型 基因组测序 序列分析 Hand-foot-mouth disease Echovirus 1 Genome sequence Phylogenetic analysis
分类号 R373.24 [医药卫生—病原生物学]
  • 相关文献

参考文献13

  • 1Zhang Y, Zhu Z, Yang W, etal. An emerging recombi- nant human enterovirus 71 responsible for the 2008 outbreak of hand foot and mouth disease in Fuyang city of China [J]. VirolJ, 2010, 171- 94.
  • 2Sun LM, Zheng HY, Zheng HZ, etal. An enterovirus 71 epidemic in Guangdong Province of China, 2008: epidemio- logical, clinical, and virogenic manifestations [J]. Jpn J In- fect Dis, 2011, 64 (1), 13 -18.
  • 3Chomel JJ, Antona D, Thouvenot D, et al. Three ECHO virus serotypes responsible for outbreak of aseptic meningitis in Rhone Alpes region, France [J]. Eur J Clin Microbiol In fect Dis, 2003, 22 (3): 191 -193.
  • 4Zhao YN, Jiang QW, Jiang RJ, etal. Echovirus 30, Jiangsu Province, China [J]. Emerg Infect Dis, 2005, 11 (4): 562- 567.
  • 5Wang JR, Tsai HP, Huang SW, et al. Laboratory diagno- sis and genetic analysis of an echovirus 30-associated out- break of aseptic meningitis in Taiwan in 2001 [-J]. J Clin Mi crohiol, 2002, 40 (12): 4439-4444.
  • 6Cheon DS, Lee J, Lee K, etal. Isolation and molecular i- dentification of eehovirus 13 isolated from patients of aseptic meningitis in Korea, 2002 [J]. J Med Virology, 2004, 73 (3): 439-442.
  • 7卫海燕,许玉玲,黄学勇,马宏,陈豪敏,许汴利.2010年7～12月肠道病毒B组河南株的血清学分布[J].病毒学报,2012,28(2):114-117. 被引量：4
  • 8Tamura K, Dudley J, Nei M, etal. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0[J].MolBiolEvol, 2007, 24 (8): 1596-1599.
  • 9Pulli T, Lankinen H, Roivainen M, etal. Antigenic sites of coxsackievirusA9 [J]. Virology, 1998, 240 (2): 202- 212.
  • 10Foo DG, Alonso S, Phoon MC, et al. Identification of neutral izing linear epitopes from the VP1 capsid protein of Enterovirus 71 using synthetic peptides [J]. Virus Res, 2007, 125 (1): 61-68.

二级参考文献16

  • 1Knowles,N.J.(2011)[DB/OL].The picornavirushome page,http://www.picornaviridae.com/entero-virus/enterovirus.htm.UK:Institute for AnimalHealth,Pirbright Laboratory.
  • 2Zhang Y,Zhu Z,Yang W,et al.An emerging recombi-nant human enterovirus 71responsible for the 2008out-break of hand foot and mouth disease in Fuyang city ofChina[J].J Virol,2010,12,7:94.
  • 3Sun L M,Zheng H Y,Zheng H Z,et al.An enterovirus71epidemic in Guangdong Province of China,2008:ep-idemiological,clinical,and virogenic manifestations[J].Jpn J Infect Dis,2011,64(1):13-18.
  • 4Chomel J J,Antona D,Thouvenot D,et al.ThreeECHO virus serotypes responsible for outbreak of asep-tic meningitis in Rhone-Alpes region,France[J].Eur JClin Microbiol Infect Dis,2003,22:191-193.
  • 5Zhao Y N,Jiang Q W,Jiang R J,et al.Echovirus 30,Jiangsu Province,China[J].Emerg Infect Dis,2005,11:562-567.
  • 6Wang J R,Tsai H P,Huang S W,et al.Laboratory di-agnosis and genetic analysis of an echovirus 30-associatedoutbreak of aseptic meningitis in Taiwan in 2001[J].JClin Microbiol,2002,40:4439-4444.
  • 7Cheon D S,Lee J,Lee K,et al.Isolation and molecularidentification of echovirus 13isolated from patients of a-septic meningitis in Korea,2002[J].J Med Virol,2004,73:439-442.
  • 8Oberste M S,Nix W A,Mahe K,et al.Improved mo-lecular identification of enteroviruses by RT-PCR andamplicon sequencing[J].J Clin Virol,2003,26(3):375-377.
  • 9Oberste M S,Maher K,Kilpatrick,et al.Typing of hu-man enteroviruses by partial sequencing of VP1[J].JClin Microbiol,1999,37(5):1288-1293.
  • 10Bingjun T,Yoshida H,Yan W,et al.Molecular typ-ing and epidemiology of non-polio enteroviruses isolatedfrom Yunnan Province,the People's Republic of China[J].J Med Virol,2008,80(4):670-679.

共引文献3

中国病毒病杂志
2013年 第5期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部