摘要
目的:了解柯萨奇病毒B5(CoxB5)河南分离株全基因特征及与其他肠道病毒基因的关系。方法:采用RT-PCR扩增全基因,采用DNAStar中的SeqMan拼接所测序列,BioEdit进行同源性比较,Simplot进行相似度分析。结果:CoxB5/Henan/2010基因组全长7401bp。与原型株Faulkner相比,编码区氨基酸同源性为95.6%,核苷酸同源性为79.9%,其中VP4-VP2核苷酸差异为17.8%～20.4%,VP1核苷酸差异为19.5%,P2、P3核苷酸差异分别为19.5%、21.7%。不同型别肠道病毒间VP4-VP2核苷酸差异为14.4%～34.9%,VP1核苷酸差异为18.3%～42.3%,P2、P3核苷酸差异为17.1%～21.0%、15.5%～22.6%。结论:与原型株相比,CoxB5/Henan/2010株编码区发生沉默突变,其核苷酸变异并不影响氨基酸序列的变化;肠道病毒基因组各分区在进化中不同步。
Aim：To reveal the genomic sequence characteristic of coxsackie virus B5（CoxB5）and its relationship with other enterovirus gene.Methods：The whole genome sequence was sequenced by RT-PCR,and BioEdit and Simplot were used to analyze the homology and similarity.Results：The full length of CoxB5/Henan/2010 genome was 7 401 bp.Compared with the prototype strain Faulkner,the entire coding region of amino acid homology was 95.6%,and VP4-VP2 nucleotide difference was 17.8%～20.4%,VP1 nucleotide difference was 19.5%,and P2,P3 nucleotide differences were 19.5%,21.7%.VP4-VP2 displayed 14.4%～34.9%,VP1 displayed 18.3%～42.3%and P2,P3 displayed 17.1%～ 21.0%,15.5%～22.6%in nucleotide difference compared with the other enterovirus,respectively.Conclusion：Compared with the prototype strain,CoxB5/Henan/2010 coding sequence is silent mutation,the nucleotide variation does not affect amino acid sequence changes.The genome evolution is not sync in the district of enterovirus.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2011年第6期868-871,共4页
Journal of Zhengzhou University(Medical Sciences)
基金
河南省医学科技重大攻关基金资助项目201001015
