摘要
采用夹心斑点杂交法、PCR—EB定性及Amp -lisensor定量法对HBsAg与抗—HBs 同时阳性、HBsAg阳性、抗—HBs 阳性和HBsAg与抗—HBs 均阴性的乙型肝炎病毒 (HBV)感染者检测血清HBVDNA。结果三种方法的阳性率分别为 2 9 1 %、4 7 7%和 80 2 % ;各组HBVDNA含量分别是 1 0 6.13± 1.86、1 0 6.0 8± 1.82 、1 0 4 .12± 1.4 0 和 1 0 5.0 7± 1.2 1(拷贝 /ml)。结论是Amplisensor法检测HBVDNA具有更高的敏感性 ;抗—HBs 阳性的HBV感染者血清中仍可检出HBVDNA。
Objective To compare the sensitivity and clinical significance of different detection methods in sera HBV DNA of patients with hepatitis B.Methods Serum HBV DNA of 26 cases of both HBsAg and HBsAb positivity(groupⅠ),20cases of HBsAg positivity and HBsAb negative(group Ⅱ),20 cases of HBsAg negative and HBsAb positivity(group Ⅲ)and 20 cases both HBsAg and HBsAb negative(group Ⅳ)was measured by double-layered blot hybridization assay,PCR-EB and Amplisensor assay respectively.Results Positivity rates of HBV DNA by three methods were 29.1%,47.7% and 80 2% respectively.Quantities of HBV DNA in group Ⅰ,group Ⅱ,group Ⅲ and group Ⅳwere 10 6.13±1 86 ,10 6.08±1 82 ,10 4.12±1 40 and 10 5.07±1.21 copies/ml respectiely.Conclusion There is higher sensitivity in method of Amplisensor assay and HBV DNA exiets in sera of some patients with HBsAb positivity.
出处
《生物学杂志》
CAS
CSCD
2000年第6期15-16,共2页
Journal of Biology
基金
美国中华医学资金会(CMB)及卫生部优秀青年人才资金!(编号 :970 1 8)提供资助
关键词
乙型肝炎病毒
血清标志物
HBV
DNA
检测
Hepatitis B
HBV DNA
Double-Layered Blot Hybridization PCR Amplisensor assa
