摘要
应用抗间日疟原虫和抗恶性疟原虫红内期抗原单克隆抗体建立了双夹心斑点免疫金银染色法和双夹心免疫酶斑点法并用于检测间日疟和恶性疟患者血清中循环抗原。共检测20份间日疟血清和15份恶性疟血清,双夹心斑点免疫金银染色法阳性率分别为90％和93％,可检出最低原虫密度为0.0009％;双夹心免疫酶斑点法阳性率分别为85％和87％,可检出最低原虫密度为0。0075％。与包虫病。弓形虫病和乙肝表面抗原阳性血清无交叉反应,检测60份健康献血员血清,两法分别有5％和8％假阳性。初步结果提示双夹心斑点免疫金银染色法检测疟疾循环抗原敏感度较高,若经过适当改进,有可能用于疟疾现场诊断和流行病学调查。
A sandwich dot-enzyme-linked immunosorbent assay (S-Dot-ELISA) and sandwich dot-immunogold silver staining assay ( S-Dot-TGSSA ) were used to detect Plasmodium vivax ( Pv ) and Plasmodium falciparum ( Pf ) circulating antigens in patient's serum with monoclonal antibodies ( McAbs)against the erythrocytic stages of Pv and Pf. Of the 20 Pv infected sera tested, 17 were positive in S-Dot-ELISA
(85%) and 18 in S-Dot-IGSSA (90%) .Of the 15 Pf sera tested, 13 were positive in S-Dot-ELISA (87% ) and 14 in S-Dot-IGSSA ( 93% ) .The minimum detectable parasitemia for Pv antigen were 0.0075% in S-Dot-ELISA and 0.0009% in S-Dot-IGSSA. There were no cross reactions with hydatidosis, toxoplasma and HBsAg positive sera. Of the 60 normal sera tested, 5 were false positive in S-Dot-ELISA
( 8 % ) and 3 in S-Dot-IGSSA ( 5% ) .The preliminary results show that the S-Dot-IGSSA has a higher sensitivity than S-Dot-ELISA in detecting malaria antigens and after proper modification the former is possible to the used in the field for the diagnosis of malaria infection.
出处
《第一军医大学学报》
CSCD
1991年第2期105-108,共4页
Journal of First Military Medical University
基金
军队青年科学基金资助
关键词
疟疾
恶性疟原虫
诊断
循环抗原
Plasmodium falciparum
Plasmodium vivax
circulating antigen
antibodies, monoclonal
Immunogold silver staining assay
Dot-ELISA
