摘要
本文以酶水平,研究了两个自然群体的遗传多样性。采用聚丙烯酰胺凝胶电泳技术,检测了长白松雌配子体的谷氨酸草酰乙酸转氨酶(GDT),苹果酸脱氢酶(MDT),酸性磷酸酶(ACP),谷氨酸脱氢酶(GDH)和葡萄糖脱氢酶(GLUDH)的同功酶型。根据多态基因位点标准,笔者发现检测基因位点的63％是多态的。8个基因位点所有样品的杂合性的平均值是0.3157。每个基因位点的平均等位基因数是2.19。两个自然群体间的同功酶多样性分化,对8个基因位点的基因多样性测量表明,群体间基因多样性的分化明显效果是9％,总的基因多样性的91％在群体内。两个群体间的遗传距离是0.0619。
Genetic deversity in two natural populations of pinus sylvestriformis was studied at the enzyme level.Using technique of polyacrylamid gel slab electrophoresis,we have determined the isoenzyme patterns of glutamate oxalo-acetate transaminase (GOT), malate dehydrogcnase (MDH), glutamate dehydrogenase (GDH), glucose dehydrogenase (GLVDH)and acid phosphates(ACP) in seed macrogamctophytes of Pinus sylvestriformis. 63% loci were detected to be polymorphic, depending on the critcrion of polymorphism. The mcan value for all samples of the average expected heterozygosity over 8 loci was 0.3157.The average number of alleles detected per locus was 2.19. Isozyme differentiated between populations.Our measurcment of gene divcrsity for the 8 loci showed 9% significant effect of interpopulation, 91% of total gene diversity rcsided within populations.The genetic distance of two populations was 0.0619.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
1991年第5期17-25,共9页
Journal of Northeast Forestry University
基金
国家自然科学基金
关键词
长白松
遗传
同功酶
Pinus sylvestriformis
Genetic diversity
Isozyme
