摘要
应用等位酶分析法,分析了18个林分群体（小群体）280多株黄山松种子胚乳的EST和MDH酶的酶基因．结果表明：EST酶受3个基因位点调控,其中2个位点各有等位基因5个、4个,平均为3.58个；MDH酶受4个基因位点调控,其中3个位点各有等位基因3个、5个、5个,平均为2．81个。基因位点均是多态的。等位基因在群体中的分布不同。EST酶基因位点的平均期望杂合度为0.562。MDH酶基因位点的平均期望杂合度为0．418。固定指数F=-0．087＜0,表明杂合体组成的小群体比例较高,杂合体过量。群体间EST酶基因的遗传距离D=0．1729,MDH酶基因的D=0．2934,群体间变异量占14％左右,表明小群体间的遗传差异较大。黄山松群体的基因分化明显，用遗传距离D值聚类，17个群体可以分成7～8类。
The genetic variation of EST and MDH allozyme genes was studied using isozyme loci analysis of seed endosperms in 18 natural stand population (separate lesser populations) of Pinus taiwanensis from 5 provinces. The results are as follows: EST enzymes are affected by 3 gene loci,two of which have a respective allele of 5 and 4,averaging 3. 58; MDH enzymes are affected by 4 gene loci,3 of which have a respective allele of 3. 5 and 5,averaging 2. 81. Gene loci are polymorphic,and alleies are unevenly distributed in different populations. The average expected heterozy gosity (He) of gene loci in EST enzymes is 0. 562,and that in MDH enzymes is 0. 418,fixation index F is -0. 087,demonstrating a higher proportion of lesser populations composed of heterozygotes;with EST enzyme,genetic distance D is 0. 1729,while with MDH enzymes D is 0. 2934,indicating a 14% of variation and a sharp difference in genetics among lesser populations. 17 populations can be divided into 7 to 8 categories based on D values.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
1999年第3期14-19,共6页
Journal of Northeast Forestry University
基金
国家自然科学基金
浙江省自然科学基金
关键词
黄山松
酶基因
基因频率
遗传距离
群体变异
Pinus taiwanensis
Enzyme gene
Gene frequency
Genetic distance
Division of population
Population variation
