摘要
在白皮松主要分布区抽取了 10个天然群体进行酶电泳分析 ,共测定了 16种酶系统 ,得到 31个清晰酶位点和 5 3个等位基因。按照基因频率进行分类 ,5 3个基因中有 32个全局基因、14个广域基因、6个局域基因和 1个特异基因。通过计算机模拟建立基因捕获曲线的结果表明 ,随机抽取 5个群体可平均捕获 99.9%的基因。群体的等位基因频率与地理生态因子的相关分析表明 ,Idh与Pgi 2两个位点的基因频率呈现明显的梯度变异。
Pinus bungeana is an endemic pine with typical fragmented distribution in central and northwestern China. Its genetic diversity is gradually decreasing and needs to be conserved. For strengthening its genetic resources conservation, ten populations were sampled in the main distribution areas of Pinus bungeana and studied by starch gel electrophoresis on 16 enzyme systems. On the basis of analysis of allozyme bands, 31 loci and 53 alleles were coded, among which, there were 32 essential genes, 14 widespread genes, six sporadic genes and one unique gene. Differences exist in the number and type of genes among populations. The central population P4 had the largest number of genes, and the marginal populations P1, P7 and P10 had the least, but they had more rare and unique genes. Sampling capture curve analysis indicated that the captured genes increased when the sampled populations increased, and 99.9% out of the whole gene pool of ten populations would be captured when five populations were randomly selected. Correlation analysis showed that gene frequency on loci Idh and Pgi 2 had gradient variation trends which were in accord with most of the phenotypic characteristics. This study provides the basis for gene conservation in natural populations of P. bungeana.
出处
《生物多样性》
CAS
CSCD
2003年第1期28-36,共9页
Biodiversity Science
基金
国家十五攻关项目"林木种质保存技术创新与利用研究"(2 0 0 1-13 )
国家基础性工作项目"林木种质资源收集保存"(19992 0 0 02 0 0 1)
