摘要
目的通过扩增乌鲁木齐维吾尔族HIV-1感染者gag基因片段,确定该民族HIV-1感染者流行株的基因亚型。方法对68例新疆乌鲁木齐市HIV-1感染者的血浆标本的RNA,用巢式PCR扩增gag基因片段,将所得到的序列与国际标准株进行比较,确定被检标本的亚型。结果在68例HIV感染者的血浆标本中,共成功扩增35例gag基因片段,扩增率为51.47%。经BLAST初步判断和构建进化树并与国际标准株比对确认,35例感染者感染的HIV毒株序列中,发现均为CRF07—BC亚型。35例毒株之间的平均基因距离为(0.043±0.003),与3例CRF07—BC亚型国际参考株之间的平均基因距离在(0.034±0.004)^(0.039±0.005)之间。结论新疆乌鲁木齐市HIV-1感染者以CRF07—BC亚型为主要流行株。35例序列相互之间以及与3株不同来源的国际参考毒株之间表现出高度的同源性。
Objective To explore the prevalent gene subtypes of HIV 1 among HIV- 1 infected patients in Urumqi Uygu nationality by amplifying gag gene. Methods HIV - 1 RNA was extracted from the plasma of 68 HIV 1 infected pa- tients in Urumqi. HIV - 1 gag gene was amplified by nested - PCR and was further subject to DNA sequencing and subtype analysis. Results The gag genes from 35 cases were successfully amplified, with an amplification rate of 51.47 %. Prelimi- nary judgment by BLAST, phylogenetic tree mapping and comparison with international standard strains indicated that all of the genes of the 35 strains were CRF07_BC subtype. The average gene distance of the 35 strains was 0. 043 ± 0. 003, The average gene distance to the 3 international standard strains ranged from 0. 034 ± 0. 004 to 0. 039 ± 0. 005. Conclusions The preva- lent subtype of H/V 1 in the HIV- 1 infected patients in Urumqi is CRF07_BC. The gene sequences of all of the strains from 35 cases and of 3 international standard strains are highly homogenous.
出处
《实用预防医学》
CAS
2013年第9期1038-1040,共3页
Practical Preventive Medicine
基金
新疆维吾尔自治区人民医院科研项目(20110130)
