摘要
目的观察静脉应用胺碘酮对急性心肌缺血大鼠心律失常和心肌梗死面积的影响。方法健康雄性SD大鼠50只，体重250-300g，采用随机数字表法将大鼠随机分为五组（n=10）：假手术组（S组）开胸后穿线做套环，但不收紧结扎绂；缺血-再灌注组（IR组）收紧结扎线缺血30min，结扎线放松行再灌注120min；A1组于缺血前10min静脉注射胺碘酮2．5mg／kg，注射后持续泵注0．15mg·kg^-1·min^-1直至再灌注末；A2组和A3组于缺血前10min静脉注射胺碘酮5．0mg／kg，其中A3组注射后持续泵注0．15mg·kg^1·min^-1直至再灌注末，观察五组大鼠心律失常评分以计算左心室（LV）、缺血危险区（AAR）和梗死区（IS）的体积，用AAR／LV和IS／AAR表示心肌梗死的程度。结果与S组比较，IR组、A1组、A2组、A3组心律失常评分明显升高（P〈0．05）；与IR组比较，A1组、A2组、A3组心律失常评分明显降低（P〈0．05），且A3组明显低于A1、A2组（P〈0．05）。S组未见心肌梗死区；与IR组比较，A1组、A2组、A3组IS／AAR明显减少（P〈0．05），且A1组、A2组和A3组IS／AAR依次递增；梗死面积IR组〉A1组〉A2组〉A3组（P〈0．05）。结论静脉注射胺碘酮可以有效减少急性心肌缺血-再灌注引起的心律失常，同时缩小心肌梗死面积，其中以5．0mg／kg静脉注射加0．15mg·kg^-1·min^-1持续泵注的给药方式效果更为明显。
Objective To explore the influence of intravenous amiodarone on acute myocardial ischemia arrhythrnias and myocardial infarct size in rats. Methods Fifty adult male SD rats, weighting 250- 300g, were randomly divided into 5 groups （n=10） ： sham operation group （group S）, IR control group： myocardial IR was produced for 30 rain isehemia followed by 120 rain reperfusion, 2.5 mg/kg amiodarone group （group A1） ： amiodarone was given 10 min before ischemia at 2. 5 mg/kg followed by 0. 15 mg/kg until the reperfusion ended; 5.0 m4g/kg amiodarone group （group A2） ： amiodarone was used 10 rain before ischerrda at 2. 5 mg/kg; 5. 0 mg/kg amiodarone group （group A3） ： amiodarone was given 10 rain before ischemia at 5 mg/kg followed by 0. 15 mg/kg until the reperfusion ended. The arrhythmias rate and the myocardial infarct size when ischemia and the infarct size in each group were recorded. Results Compared with the group S, the arrhythmias scales of acute ischemia in group IR, A1, A2, A3 were significantly lower （P〈0. 05）. Compared with group ｜R, the arrhythlnias scales of acute ischemia in group A1, A2 and A3 were significantly lower （P〈0. 05）. The arrhythmias scales of acute ischernia was reduced gradually, compare to the groups A1 and A2, the arrhythmias scales of acute ischemia in group A3 was reduced more sharply, which had statistical difference （P〈0.05）. There was no infarct area in the group S, the infarct size in amiodarone-treated groups were significantly reduced compared with IR group, while in group A1, the infarct size was larger than in group A2 and A3 （P〈0.05）, but in the group A3, the infarct size was reduced much more than in group A2, comparison between them had statistic difference Conclusion 5.0 mg/kg amiodarone with maintenance dose of 0. 15 mg.kg-1 .min-1 can reduce the arrhythmias induced by acute ischemia as well as reduce the infarct size.
出处
《临床麻醉学杂志》
CAS
CSCD
北大核心
2013年第9期905-907,共3页
Journal of Clinical Anesthesiology
基金
宁夏自然科学基金(nx11185)
关键词
胺碘酮
缺血-再灌注损伤
心律失常
心肌梗死
Amiodarone
Ischemia-reperfusion injury~ Arrhythmia
Myocardial infarct
