摘要
目的:研究观察七味温阳软胶囊对肾阳虚模型动物垂体-肾上腺皮质系统、垂体-性腺系统功能及应激反应的影响。方法:将60只Wistar种大鼠、78只昆明种小鼠,分别按性别体重均衡随机分为正常对照组,模型对照组,阳性药对照组,七味温阳软胶囊高、中、低剂量组。大鼠从给药第1天起,除正常对照组外,各组均ig氢化可的松5×10-5g·kg-1每天1次,连续9 d,制造肾阳虚模型,继续给药至第12天,各组动物股动脉取血,离心,取血清,检测促肾上腺皮质激素(ACTH)、皮质醇(Cortisol)水平;小鼠连续给药14 d,于给药的第8至14天时,除正常对照组外,每只ig氢化可的松0.75 mg·kg-1,每天1次,连续7 d,末次给药后检测小鼠包皮腺、精液囊脏器指数及小鼠游泳和耐缺氧时间。结果:七味温阳软胶囊0.96,0.48 g·kg-12个剂量可明显升高肾阳虚模型大鼠血清ACTH,Cortisol水平(P<0.05);1.35,0.68 g·kg-12个剂量可明显增加肾阳虚小鼠包皮腺脏器指数(P<0.05);0.68,0.34 g·kg-12个剂量可明显增加肾阳虚小鼠精液囊脏器指数(P<0.05,P<0.01);1.35,0.68,0.34 g·kg-13个剂量均可明显延长小鼠游泳时间和常压耐缺氧时间(P<0.05)。结论:七味温阳软胶囊对肾阳虚模型大鼠垂体-肾上腺系统功能和小鼠垂体-性腺系统功能具有一定的保护作用。
Objective： To observe effect of Qiwei Wenyang softcapsule on pituitary-adrenal cortex system and pituitary-gonadal system function in animal kidney Yang deficiency model. Method： The high dose of hydrocortisone was used to establish the kidney Yang deficiency model, at the same time, Qiwei Wenyang soft capsule was given, and serum corticotrophin （ACTH） and cortisol level preputial gland, seminal vesicle organ index, the mice swimming time and hypoxia tolerance time were observed. Result： The high and medium dose Qiwei Wenyang softcapsule （0. 96, 0. 48 g·kg^-1） could obviously increase the serum ACTH and cortisol levels （P 〈0. 05） in rats, 1 and preputial gland organ index in mice （P 〈 0. 05）, Qiwei Wenyang softcapsule at dose of 0. 68, 0. 34 g ·kg^-1 could obviously increase seminal vesicle organ index （P 〈 0. 05 or P 〈 0. 01 ） ; significantly prolong the swimming time and the normal pressure hypoxia tolerance time at dose of 1.35, 0. 68, 0. 34 g·kg^-1 of mice （P 〈 0. 05）. Conclusion： Qiwei Wenyang softcapsule has a protective effect on kidney Yang deficiency model.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第19期242-244,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
兰州市生物医药专项(2012-2-75)
关键词
七味温阳软胶囊
肾阳虚
补肾温阳
Qiwei Wenyang softcapsule
kidney Yang deficiency
Bushen Wenyang
