摘要
目的:探讨三叶香茶菜防治大鼠肝纤维化的作用机制。方法:大鼠每3 d接受一次背部sc 40%CCl4花生油(首次5 mL·kg-1,其余为3 mL·kg-1),连续8周引发肝纤维化模型,造模同时每日分别给予三叶香茶菜提取物20,40,80 g·kg-1灌胃。给药8周后取血,ELISA法测定大鼠血清中Ⅲ型前胶原(proeollagen typeⅢ,PCIII)、透明质酸(hyaluronic acid,HA)、金属蛋白酶组织抑制因子-1(tissue inhibitor of metalloproteinase-1,TIMP-1)、基质金属蛋白酶-2(matrix metal oproteinase-2,MMP-2)及肝脏中转化生长因子-β1(transforming growth Factor-β1,TGF-β1)含量;Masson染色病理切片观察肝脏胶原纤维生成。结果:模型组血清中HA,PCIII,TGF-1及TIMP-1含量较正常组显著升高(P<0.01),且MMP-2浓度显著下降(P<0.01)。与模型组比较,三叶香茶菜高、中剂量组能显著降低大鼠血清HA,PCIII,TIMP-1的含量,提高MMP-2含量(P<0.01);并能显著降低肝组织TGF-β1水平(P<0.01)。三叶香茶菜低剂量组能显著降低大鼠血清HA,PCIII含量(P<0.05);并有降低血清TIMP-1及肝组织TGF-β1水平,提高MMP-2含量的趋势。结论:三叶香茶菜能有效减轻慢性肝损伤大鼠肝纤维化程度,其作用机制可能与调节TGF-β1水平,控制肝星状细胞释放TIMP-1及MMP-2有关。
Objective： To investigate the mechanism of Rabdosia ternifolia on hepatic fibrosis in rats. Method： Rat hepatic fibrosis model was produced by back subcutaneous injection of 40% carbon tetrachloride （CC14） peanut oil, once every three days, for eight consecutive weeks, at the same time the rats were given R. ternifolia extract at 20, 40, 80 g ·kg^-1 with gavage. Take blood After 8 weeks of administration, serum were collect, and the ELISA method was used for the determination of serum proeollagen type m （PCIII） , hyaluronic acid （HA）, tissue inhibitor of metalloproteinase-1 （TIMP-1）, matrix metal oproteinase-2 （MMP-2） and liver transforming growth factor-ill （TGF-β1） concentration. The liver collagen fiber formation was observed by Masson staining. Result： Serum HA, PCIII, TGF-β1and TIMP-1 concentration were significantly raised in model group rats than normal group rats （P 〈 0.01 ）, while MMP-2 was significantly decreased （P 〈 0.01 ）. R. ternifolia at high, medium dosage significantly reduced the content of HA, PCIII, TIMP-1, as well as increased the content of MMP-2 in serum （P 〈 0. 01 ） ; and the extract significantly reduced TGF-β1 level in liver （P 〈 0. 01 ）. The R. ternifolia at low dosage significantly reduces the levels of serum HA, PCIII content （ P 〈 0.05 ） ; it also demonstrated a trend of decreasing serum TIMP-1 and liver TGF-β1 content, increasing of the content of MMP-2, but the difference with the model group is not significant. Conclusion： R. ternifolia effectively alleviated the degree of the chronic liver injury in liver fibrosis rat, the mechanism may be related to the regulation of TGF-β1 level and the controlling of the release of the TIMP-1 and MMP-2 in hepatic stellate cells.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第19期238-241,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广西教育厅科研项目(201012MS152)
广西科技基础条件平台建设项目<中药药效研究重点实验室建设>(10-046-04-Z16)
广西自然科学基金创新研究团队项目(2011GXNSFF018006)
关键词
三叶香茶菜
肝纤维化
四氯化碳
Rabdosia ternifolia
liver fibrosis
carbon tetrachloride
