摘要
目的评价福建叶下珠对小鼠免疫性肝损伤的保护作用。方法48只NIH小鼠随机分为叶下珠高、低剂量组、联苯双酯组、正常对照组及模型组。于造模之日起,叶下珠高、低剂量组分别以20 g/kg、10 g/kg叶下珠药液灌胃,联苯双酯组以0.15 g/kg联苯双酯药液灌胃,正常对照组及模型组每天给予等量生理盐水。每天灌胃1次,连用12 d。检测血清谷丙转氨酶(ALT)和肝、脾脏器指数,并做肝脏病理学检查。结果联苯双酯组、叶下珠高、低剂量组小鼠血清ALT、肝指数、脾指数值较模型组有不同程度的改善(P<0.05,P<0.01)。炎症坏死程度:5组间比较,差异有非常显著性意义(P<0.005);模型组与正常组、联苯双酯组、福建叶下珠高、低剂量组与模型组间两两比较,差异有显著性意义(P值均<0.05);联苯双酯组与福建叶下珠大、小剂量组间比较,差异无显著性意义(P>0.05)。结论福建叶下珠能显著降低血清转氨酶活力和肝脾脏器指数,对肝细胞坏死有一定的改善作用,具有较好的抗小鼠免疫性肝损伤作用。
Objective To evaluate the protective action of Phyllanthus orinaria （PU） for immune liver injury in mice. Methods Forty-eight NIH mice were allocated randomly to high-dose （HG） PU group, low-dose （LG） PU group, bifenbate group （BG）, normal control group （NCG）, and model group （MG）. From the first day of establishing the models, 20 g · kg^-1 of PU solution was given for gastric perfusion （GP） to HG PU group, 10 g · kg^-1 of PU solution for GP to LG PU group,0.15 g·kg^-1 of bifenbate solution for GP to BG group, and the equal amount of normal saline to the normal control group and the model group, qd for 12 days. Serum ALT level and visceral parameters of liver and spleen were determined, and the liver pathological feature was also examined. Results The ALT level, parameters of liver and spleen were markedly decreased in BG group （P 〈 0. 01 compared with the model group）, and improved accordingly in HG and LG PU groups in comparison with the model group （P 〈 0. 05, P 〈 0. 01 ）. The comparison of inflammatory necrosis among 5 group showed very markedly significant difference （P 〈 0. 005） ; the paired comparison of MG and NCG, that of BG, HG PU and LG PU with MG showed markedly significant difference （P 〈 0.05）; the comparison of BG with HG PU and LG PU showed no significant difference （P 〉 0. 05）. Conclusion PU has a good effect on counteracting the immune live injury in mice by markedly decreasing the activity of serum transaminase and visceral parameters of liver and spleen, and improving the necrosis of hepatic cells.
出处
《中药新药与临床药理》
CAS
CSCD
2005年第5期343-345,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
关键词
福建叶下珠
免疫性肝损伤
卡介苗
脂多糖
小鼠
Phyllanthus urinaria
Immune liver injury
Bacillus Calmette - Guerin vaccine
Lipopolysaccharide
Mice
