摘要
用DNA拓扑异构酶I抑制剂-喜树硷体外诱导人低分化鼻咽癌上皮细胞系（CNE－2Z）细胞发生凋亡，并用流式细胞仪检测了有关癌基因表达的变化情况。结果：4μmol／L喜树俭作用24h后，c－myc、bcl－2及p53表达均较对照组明显降低，提示CNE－2Z细胞发生凋亡时或许会受c－myc、bcl－2和p53等癌基因的影响。
Topoisomerase I inhibitor-camptothecin was used to induce apoptosis of human nasopharyngeal carcinoma cell line (CNE-2Z), and flow cytometry was used to determine some oncogene expressions associated with apoptosis. The results showed that expressions of c-myc.bcl-2 and p53 declined obviously in CNE-2Z cells after being treated with 4 μmol/L CPT for 24h. Statistical analysis indicated that there were significant differences in the positive ratio between the controls and the treatment groups, which suggested that c-myc, bcl-2 and ph3 might be involved in the apoptosis of CNE-2Z cells induced by camptothecin.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
1998年第2期168-172,共5页
Chinese Journal of Histochemistry and Cytochemistry
关键词
鼻咽癌
枣树硷
细胞凋亡
癌基因
Nasopharyngeal carcinoma, Camptothecin. Apoptosis, Oncogene
