摘要
目的总结归纳知母皂苷类化合物的质谱裂解规律。方法通过查阅分析近年来国内外文献,结合本实验室研究工作,整理出46种知母皂苷类成分化学结构信息,并比较它们在正负离子模式下的质谱裂解途径及碎片离子。结果螺甾烷醇类皂苷首先丢失C-3位糖链上的各单糖,然后E环断裂脱去侧链(如144u和114u),最后生成苷元的特征碎片离子m/z255或253或251、m/z285或283或281;而呋甾烷醇类皂苷首先脱去C-22位的—OH/—OMe后出现[M+H-H2O]+/[M+H-MeOH]+的分子离子峰,然后丢失C-26位糖链,其进一步的裂解规律与螺甾烷醇类皂苷类似。结论正负离子模式下的质谱裂解规律及其它们的特征碎片离子将有助于知母皂苷类化合物的结构解析。
Objective To summarize the fragmentation pathways of spirostanol saponins and furostanol sapo- nins. Methods The chemical structures and the characteristic fragment ions of 46 saponins in Anemarrhenaasphodeloides were reorganized. Results It was found that the spirostanol saponins firstly lose C-3 sugar moiety, and then the characteristic fragmentation involving the loss of 144 u or 114 u which arise from the cleavage of the E-ring is observed. The process results in the generation of m/z 255 or 285, m/z 253 or 283, m/z 251 or 281. When --OH or --OMe is substituted at C-22 position, the furostanol saponins usually yield [M ＋H-H2O3 ions or [M ＋H - MeOH3 ions. Successively,the [M ＋H -H2O] ions or[M ＋H - MeOH] ~ ions generate product ions because of the loss of C-26 sugar moiety. The further fragmentation pathways are the same as the spirostanol saponins＇. Conclusions The fragmentation pathways and characteris- tic fragment ions will be helpful in identifying the structures of saponins in A. asphodeloides.
出处
《沈阳药科大学学报》
CAS
CSCD
北大核心
2013年第9期740-748,共9页
Journal of Shenyang Pharmaceutical University
基金
国家科技重大专项基金资助项目--重大新药创制(2012ZX09301001-001)
关键词
知母
螺甾烷醇类皂苷
呋甾烷醇类皂苷
质谱裂解规律
Anemarrhena asphodeloides Bunge.
spirostanol saponins
furostanol saponins
fragmentationpathways
