摘要
采用直接中和法,在乳酸菌发酵液中加入碳酸钙进行中和反应。通过对乳酸菌富集培养和发酵条件的优化提高发酵产酸量。单因素试验确定适宜培养条件为:接种量8%,装液量20%,温度36℃,初始pH值为6.0。用正交试验法对其发酵条件进行优化,最佳优化条件为:温度40℃,时间96h,接种量8%,碳源10%,初始pH值为7.0。再通过浓缩结晶法提取乳酸钙,最终乳酸钙收率可达72.10%。此工艺为后续乳酸菌发酵法回收利用含糖废液提供了实验基础和理论依据。
In the article, direct neutralization was employed to isolate the lactic acid bacteria. The calcium carbonate was added in the lactic acid fer- mentation liquid. The enrichment culture of lactic acid bacteria and optimization of fermentation conditions were used to improve fermentation yield. Appropriate culture conditions conftrmed by single factor experiments were as follows： the inoculum 8%, the loaded liquid 20%, the temperature 36℃ and the initial pH value 6.0. Optimum fermentation conditions optimized by orthogonal experiment were as follow： the temperature 40℃, the fermentation time 96h, inoculum 8%, carbon source 10% and the initial pH value 7.0. By re-crystallization and concentrating method to extract calci- um lactate, the yield was up to 72.10% finally. This process provided the experimental and theoretical basis for the following lactic acid fermentation recycling of waste sugar.
出处
《中国酿造》
CAS
2013年第8期99-102,共4页
China Brewing
关键词
乳酸菌
发酵条件
优化
正交试验
乳酸钙
lactic acid bacteria
fermentation conditions
optimization
orthogonal experiment
calcium lactate
