摘要
目的:建立同时测定泸州龙眼肉、核、皮中没食子酸和鞣花酸含量的HPLC方法。方法:依利特Sino Chrom ODS-BP(4.6 mm×250 mm,5μm),流动相乙腈-0.1%磷酸溶液,梯度洗脱,改变波长测定(0～11.5 min,215 nm,测定没食子酸;11.5～25 min,255 nm,测定鞣花酸),柱温30℃,流速1 mL.min-1,进样量10μL。结果:没食子酸在0.229～2.29μg,鞣花酸在0.412 5～4.125μg峰面积与进样量有良好的线性关系(r=0.999 9),平均回收率(n=9)分别为98.27%(RSD 0.63%),98.31%(RSD 0.88%)。结论:该方法灵敏、准确、重复性好,可用于泸州龙眼中没食子酸和鞣花酸的含量测定。
Objective： To establish a method for determination of the gallic gallic acid and ellagic ellagic acid indifferent part of Dimocarpus longan. Method： An HPLC method was performed on a SinoChrom ODS-BP （4.6 mm ×250 mm, 5 μm） at 30 ℃. The mobile phase was acetonitrile-0. 1% phosphoric acid with gradient elution and changeable wavelength detection （0-11.5 min, 215 nm for gallic acid and 11.5-25 min, 255 nm for ellagic acid）. The flow rate was 0. 8 mL · min-1 and the sample size was 10μL. Result： The calibration curve of gallic gallic acid and ellagic ellagic acid showed good linearity over the range of 0. 229-2.29μg （ r = 0. 999 9 ） , 0.4125-4.125 μg （r =0.9999）. The average recoveries were 98.27% （RSD 0.63%）, 98.31% （RSD 0.88% ）. Conclusion： This method is sensitive, accurate, reproducible, it can be used for the quality control of longan.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第6期84-86,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
泸州市科技局指导性项目(泸市科[2007]64号)
