摘要
目的 建立宜宾地区健康人群血清尿素和肌酐的参考区间.方法 收集2012年4月～7月体检健康者1 919名（年龄7月～90岁,其中男982名,女937名）,并按性别年龄分组,采集空腹静脉血液后离心,血清置于-34℃冰冻保存,用日立7600-020全自动生化分析仪同一天集中测定血清尿素（谷氨酸脱氢酶法）和肌酐（肌氨酸氧化酶法）含量,通过严格的质量管理措施以保证检测结果的准确性.以正态分布法建立各年龄阶段血清尿素肌酐参考区间.结果 各组间结果存在差异（男性尿素F=63.8,肌酐F=1159.4;女性尿素F=57.25,肌酐F=613.3,P均〈0.001）,需要结合年龄与性别划分参考区间.7月~6岁尿素参考区间为0.71~5.89 mmol/L,肌酐参考区间为11.9~39.3 μmol/L;7岁~12岁尿素参考区间为1.51~6.13 mmol/L,肌酐参考区间为23.1~57.9 μmol/L;13岁~18岁男女间尿素与肌酐差异有统计学意义（P均〈0.001）,尿素参考区间为男1.58~6.76 mmol/L,女1.52~6.14 mmol/L,肌酐参考区间为男35.3~86.3μmol/L,女32.9~68.1 μmol/L;19岁~90岁男女间尿素与肌酐差异有统计学意义（P均〈0.001）,尿素参考区间为男2.85~7.21 mmol/L,女2.39~7.29 mmol/L,肌酐参考区间为男61.7~101.7 μmol/L,女42.0~81.21 μmol/L.结论 本实验建立了宜宾地区健康汉族人群血清尿素（谷氨酸脱氢酶法）和肌酐（肌氨酸氧化酶法）参考区间,帮助早期评判肾脏功能损伤具有重要意义.
Objective To establish the reference intervals for serum urea and creatinine in healthy individuals in Yibin. Meth- ods 1 919 healthy individuals（982 men and 937 women） aged from 7 months to 90 years old were enrolled. Blood specimens were collected and centrifuged, all serum samples were kept refrigerated below --34%. Serum urea and creatinine were measured on the Modular Pautomatic biochemical analyzer on the same day. Reference intervals were calculated using normal distribution methods. Results There were some difference among 7 groups（male urea F= 63.8,creatinine F= 1 159.4;fe- mal urea F=57.25 ,creatinine F= 613.3, P〈0. 001）. In 7 months to 6 years old group, reference intervals for serum urea and creatinine were 0. 71~5.89 mmol/L and 11.9~39.3 μmol/L respectively. In 7 to 12 years old group,they were 0. 71~ 5.89 mmol/L and 11.9~39.3 μmol/L respectively. In 13 to 18 years old group,there were significant difference between males and females. Reference interval for serum urea was 1.58~6.76 mmol/L in males and 1.52~6.14 mmol/L in females. Reference interval for serum creatinine was 35.3~86.3 μmol/L in males and 32.9~68.1 μmol/L in females. In 19 to 90 years old group, there were significant difference between males and females. Reference interval for serum urea was 2.85 7.21 mmol/L in males and 2.39~7.29 mmol/L in females. Reference interval for serum creatinine was 61.7~101.7 tzmol/ L in males and 42.0~81.21 μmol/L in females. Conclusion The reference intervals for serum urea and creatinine were es- tablished in healthy individuals in Yibin. It is of great significance for clinical diagnosis of kidney damage early.
出处
《现代检验医学杂志》
CAS
2013年第4期79-82,共4页
Journal of Modern Laboratory Medicine
关键词
健康人群
尿素
肌酐
参考区间
healthy individual
urea
creatinine
reference interval
