摘要
为满足栽培和种质保护的需求,以山苦菜的微型根茎为材料,采用试管苗培养的方法,进行了微型根茎的瓶内培养、苗床扦插和移植等研究。结果表明,以颗粒状炉灰渣为培养基的支持物,使用1/4MS+IAA 0.2 mg·L-1+蔗糖20 g·L-1作为培养基对试管苗进行微型根茎瓶内繁殖最佳;具有2个生长点的微型根茎段,在以炉灰渣为基质的温室苗床上进行扦插繁殖,是理想的繁殖方法。微型根茎试管苗移植的成活率为98.6%。移植成活的微型根茎试管苗保持了山苦菜的植物学性状。
We performed miniature rhizome culture in a bottle, miniature rootstock cutting of seedbed and tube seedling transplanting of miniature rootstock with the method of tube culture and micro rootstocks of Ixeris chinensis as materials to satisfy the requirements of planting and seed quality preservation. Results show that I/4MS ＋ IAA0.2 mg ·L^-1 ＋ sucrose 20 g·L^-1with cinder as a support is the best medium for bottle reproduction of tube seedlings. Cutting of micro rootstock with 2 growing points on the seedbed with cinder as matrix is an ideal reproduction method. The transplanting survival rate of the tube seedlings of micro rhizome is 98.6%. The survival tube seedlings preserve all botany characteristics of wild Ixeris chinensis.
出处
《山东科学》
CAS
2013年第4期28-31,共4页
Shandong Science
基金
辽宁省普通高等教育本科教学改革研究项目(201203041-4)
关键词
山苦菜
试管苗
微型根茎
Ixeris chinensis
tube seedlings
micro rootstock
