摘要
[目的]建立车前高效离体植株再生体系,为车前快速扩繁奠定基础。[方法]以车前无菌苗的下胚轴和子叶为外植体,以MS为基本培养基,研究了6-BA、NAA不同配比的培养基对车前下胚轴和子叶不定芽诱导的影响。[结果]MS培养基添加6-BA2 mg/L,5～7d的下胚轴不经过愈伤组织诱导阶段便可直接形成不定芽,不定芽诱导率为80%,每个外植体可产生7～8个不定芽。培养基中添加NAA促进不定根的分化,但影响不定芽的诱导。6-BA与NAA配合使用时,外植体易分化愈伤组织。在添加NAA的MS培养基上,再生苗诱导生根频率为100%。将再生植株移栽于盆土中,可获得100%存活率,且生长良好。[结论]6-BA促进车前下胚轴直接分化不定芽,而NAA则影响不定芽的诱导,在不定芽诱导培养基中应避免添加NAA。
[Objective] The research aimed to establish the high-effective plant regeneration system in vitro in Plantago asiatica L. and lay the foundation of rape propagation of P. asiaticca. [Method] With the hypecotyls and leaves of P. asiatica aseptic seedlings as tested materials and MS as basic medium, the effect of the media with different matching of 6-BA and NAA on the induction of adventitious buds of hypocotyls and leaves of P.asiatica. [Result] The hypocotyls cultured on the MS medium with 2mg/L 6-BA alone for 5～7dcould directly differentiate into the adventitious buds without passing through callus induction stage and the induction of adventitious buds was 80 % and an average of 7～8 adventitious buds were obtained per explant. Adding NAA on the MS medium could promote adventitious root differentiation, but influence adventitious bud induction, When 6-BA was combined with NAA, the explants were easy to differentiate into callus. The rooting induction of regenerated seedlings cultured on the MS medium with NAA was 100 %. When the regenerated plants were transplanted into pots they could obtain the survival rate of 100 % and grow well. [Conclusion] 6-BA can promote hypocotyls ofP. asiat/ca to directly differentiate into adventitious buds and NAA can influence adventitious bud induction, so NAA should be avoided to add on the medium of adventitious bud induction.
出处
《安徽农业科学》
CAS
北大核心
2008年第5期1810-1811,共2页
Journal of Anhui Agricultural Sciences
关键词
车前
下胚轴
子叶
不定芽直接诱导
组织培养
Plantago asiatica L.
Hypocotyls
Leaves
Direct induction of adventitious buds
