摘要
目的观察anti-miR-155反义寡核苷酸(AMOs)对A549细胞Wee1表达的影响。方法采用特异性anti-miR-155反义寡核苷酸抑制A549细胞内成熟miR-155的活性,realtime quantitive RT-PCR测定Wee1mRNA表达量,Western blot测定Wee1蛋白和Phospho-cdc2(Tyr15)蛋白的表达量。结果 100nmol/L浓度的AMOs处理的A549细胞中Wee1mRNA表达量与对照组A549细胞中Wee1mRNA表达量相比无显著性差异(P>0.05);与对照组相比,100nmol/L浓度的AMOs处理的A549细胞Wee1蛋白和Phospho-cdc2(Tyr15)蛋白的表达量显著增加(P<0.05)。结论采用AMOs抑制A549细胞内高水平表达miR-155活性后,可显著增强Wee1蛋白的表达。
Objective To investigate the effect of anti-miR-155 oligonucleotides on Wee1 expression in A549 cells. Methods Anti-miR-155 oligonucleotides （AMOs） were used to inhibit the activity of mature miR-155 in A549 cells. Ex- pression level of Weel mRNA was measured by realtime quantitive RT-PCR. Expression levels of Weel protein and Phospho-cdc2 （Tyrl5） protein were measured by Western blot. Results AMOs at the concentration of 100nmol/L did not exert significant effect on expression level of Wee1 mRNA. Compared with control group, Weel expression and Phospho-cdc2 （Tyr15） expression in A549 cells treated with 100nmol/L AMOs was significantly increased. Conclusion After inhibition of activity of mature miR-155 by AMOs, expression of Weel protein can be significantly enhanced.
出处
《西部医学》
2013年第9期1298-1300,1303,共4页
Medical Journal of West China
基金
国家自然科学基金资助项目(30672076)
国家重点基础研究发展"863"计划资助项目(2007AA02Z129)
