摘要
以47号山药脱毒试管苗诱导的零余子为材料,研究了光质和培养基组合对愈伤组织诱导形成时间及诱导率的影响,再将其培养在最佳培养基中,研究不同光质对愈伤组织鲜重、干重和生长率的影响.结果表明,从愈伤组织诱导形成的时间和诱导率方面,红光与MS+6-BA 2mg.L-1+2,4-D 2mg.L-1组合最佳,第6d开始形成愈伤组织,30d时愈伤组织诱导率达到100%;从愈伤组织的生长方面,白光和红光有利于愈伤组织鲜重的增加,白光和黄光有利于愈伤组织干物质的积累,红光和黄光有利于愈伤组织生长率的提高;而绿光则起了抑制作用.
The bulbils of virus-free Dioscorea opposita Thunb.cv.No.47in vitro was used to study the effects of the combination of light quality and medium on callus＇induction time and induction ratio.Then we put it in the best medium to analyze effects of different light quality on their fresh weigh,dry weight and growth rate.The results show that,The combination of the red light and the MS＋6-BA2mg.L-1＋2,4-D 2mg.L-1 are beneficial to the induction time and induction ratio of callus.And for six days later,callus appeared and thirty days later,induction ratio can reach 100%;from the growth of callus,white light and red light are conducive to increase in fresh weight,white light and yellow light are conductive to accumulation of dry weight,red light and yellow light are beneficial to the improvement of the growth rate;While green played the role of inhibition.
出处
《河南师范大学学报（自然科学版）》
CAS
北大核心
2013年第4期145-148,共4页
Journal of Henan Normal University(Natural Science Edition)
基金
河南省科技创新杰出人才计划项目(114200510013)
河南师范大学青年科学基金(2011QK18)
河南师范大学研究生科研创新项目(YL201315)
国家级大学生创新创零余业训练计划项目(201210476036)
关键词
光质
零余子
愈伤组织
诱导率
鲜重
干重
生长率
light quality
bulbil
callus
induction ratio
fresh weight
dry weight
growth rate
