摘要
目的 探讨鼻息肉中白介素 8(interleukin 8,IL 8)的表达及作用。方法 应用免疫组化和酶联免疫吸附测定法 ,对 2 2例鼻息肉和 10例对照组 (鼻中隔偏曲者 )下鼻甲组织内IL 8的分布及含量进行测定。结果 IL 8免疫阳性染色物呈棕黄色颗粒 ,主要位于鼻息肉上皮细胞浆及炎性细胞浆 ,鼻息肉组织中IL 8的含量较对照组明显增高 ,鼻息肉组的吸收值范围在 32 2 0 6～ 2 0 91 41pg/ml之间 ,对照组的吸收值范围在 2 9 31～ 332 19pg/ml之间 ,2组之间差异有显著性 (P <0 0 0 1)。结论 IL 8可能是鼻息肉发病机理中诸多因素之一 ,深入了解这一作用对寻找鼻息肉有效的治疗方法可能有重要意义。
Objective To investigate the distribution and content of immunoreaction interleukin-8(IL-8)antigen in the human nasal polyps Methods Twenty-two cases of nasal polyp and 10 cases of normal inferior turbinate were studied with immunohistochemical SABC method and ELISA method Results Immunohistochemical results demonstrated that IL-8 antigen staining occurred predominantly within inflammatory and epithelium cell plasm The level of IL-8 in nasal polyp tissue was higher than in normal control specimens The value in nasal polyp tissue was in 322 06-2091 41 pg/ml, but in normal with a range of 29 31-332 19 pg/ml The results demonstrated a remarkable difference between the two groups ( P <0 001) Conclusion These data demonstrated the presence of IL-8 antigen in nasal polyps Thus IL-8 likely plays a significant role in the pathogenesis of this disease process There is an important significance of finding a potential treatment method
出处
《中华耳鼻咽喉科杂志》
CSCD
2000年第4期255-256,I017,共3页
Chinese Journal of Otorhinolaryngology
关键词
鼻息肉
白细胞介素-8
免疫组织化学
ELISA
Nasal polyps
Interleukin-8
Immunohistochemistry
Enzyme-linked immunosorbent assay
