摘要
目的 :建立HDV /HBV感染人胎肝细胞体外培养系统。方法 :利用HDV/HBV阳性血清同时感染体外培养的人胎肝细胞 ;应用ELISA、免疫组化法、原位杂交法和斑点法检测上清液和细胞中HBsAg、HDAg、HBVDNA和HDVcDNA。 结果 :上清液和细胞中HBsAg、HDAg、HBVDNA和HDVcDNA在感染后第 2天至第 16天均可测出 ,其中上清液中HBsAg、HDAg以感染后第 4天至第 12天达高峰。 结论 :HDV在原代培养人胎肝细胞中能稳定复制和表达至少达 12d。
Objective: To establish an in vitro culture system of HDV/HBV infected primary human fetal hepatocytes Methods: The cultured primary human fetal heptocytes were simultaneously infected with HDV/HBV positive serum in vitro HDAg, HBsAg, HDV cDNA and HBV DNA were detected with ELISA, immunohistochemistry, in situ hybridization and dot blot hybridization in supernatant and cells Results: HDAg, HBsAg, HDV cDNA and HBV DNA were detected from the 2nd day to the 16th day after infection in supernatant and cells Secretion of HDAg and HBsAg reached peaks from the 4th day to the 12th day after the infection in the supernatant Conclusion: Primary human fetal hepatocytes are competent for infection with HDV/HBV HDV and HBV can steadily replicate and express in HDV/HBV infected human fetal hepatocytes and the replication and expression can at least last for 12 days
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2000年第3期224-226,共3页
Journal of Third Military Medical University
基金
国家自然科学基金!资助项目 (39570 1 66)
