摘要
封闭和不封闭ＢＡＬＢ／ｃ小鼠库普弗细胞（从尾静脉注射ＧｄＣｌ３或生理盐水２７ｍｌ／ｋｇ体重）４８ｈ后，夹闭肠系膜上动脉１ｈ后松夹，复制肠缺血再灌注模型。运用原位细胞ＤＮＡ末端标记（ＴＵＮＥＬ）和流式细胞术检测缺血前、缺血６０ｍｉｎ、再灌注３０、６０ｍｉｎ肝、脾、胸腺细胞的凋亡情况。结果肠缺血６０ｍｉｎ、再灌注３０、６０ｍｉｎ时，肝、脾、胸腺细胞凋亡数增多，封闭库普弗细胞后，其凋亡数增多更显著。表明库普弗细胞在肠缺血再灌注的肝、脾、胸腺细胞凋亡中起重要作用；库普弗细胞可能参与多器官功能不全时免疫功能紊乱的调节。
Kupffer cells of BALB/c mice were blocked by injecting GdCl 3 or 0.9% NaCl (7 mg/kg) via tail vein. Forty eight hours later, the superior mesenteric artery was occluded for 60 min followed by declamping to reproduce an intestinal ischemia re perfusion model. TUNEL (TdT mediated dUTP nick end labeling) and flow cytometry were used to detect apoptosis of hepatocytes, splenocytes, and thymocytes. Apoptosis of these cells was increased at 60 min after ischemia, and 30 min and 60 min after reperfusion, and it was even more significantly increased after Kupffer cells blockage with GdCl3.It indicates that Kupffer cells may play an important role in the apoptosis of hepatocytes, splenocytes, and thymocytes following intestinal ischemia repefusion. Kupffer cells might paticipate in modulation of the disturbance of immune system function in multiple organ dysfunction.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
1999年第2期86-87,共2页
Medical Journal of Chinese People's Liberation Army
